Mouse LDLR ELISA Kit | LDLR elisa kit

Mouse LDLR PicoKine ELISA Kit

Cat. Num. AAA178572

• Gene Names: Ldlr; Hlb301
• Reactivity: Mouse
• Synonyms: LDLR; Mouse LDLR PicoKine ELISA Kit; FH; FHC; LDL R; LDL receptor; LDLCQ2; P35951; Low-density lipoprotein receptor; LDLR elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse LDLR
• Sequence Length: 810

• Samples: Cell culture supernates, serum, plasma(heparin) and urine.
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <12pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

(Mouse LDLR PicoKine ELISA Kit standard curve )

Related Product Information for LDLR elisa kit

Principle of the Assay: Mouse LDLR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LDLR has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: A22-R790) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LDLR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse LDLR amount of sample captured in plate.

Background/Introduction: The Low-Density Lipoprotein (LDL) Receptor is a mosaic protein of 839 amino acids (after removal of 21-amino acid signal peptide) that mediates the endocytosis of cholesterol-rich LDL. In humans, the LDL receptor protein is encoded by the LDLR gene. It belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 358030301
• NCBI GeneID: 16835
• NCBI Accession #: NP_001239587.1
• NCBI GenBank Nucleotide #: NM_001252658.1
• UniProt Accession #: P35951; Q6GTJ9
• Molecular Weight: 94,947 Da
• NCBI Official Full Name: low-density lipoprotein receptor isoform 2
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: Ldlr
• NCBI Official Synonym Symbols: Hlb301
• NCBI Protein Information: low-density lipoprotein receptor
• UniProt Protein Name: Low-density lipoprotein receptor
• Protein Family: LDLR chaperone
• UniProt Gene Name: Ldlr
• UniProt Synonym Gene Names: LDL receptor
• UniProt Entry Name: LDLR_MOUSE

Uniprot Description

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Cell surface; Membrane protein, integral

Cellular Component: apical part of cell; basolateral plasma membrane; caveola; cell surface; coated pit; early endosome; endosome; external side of plasma membrane; Golgi apparatus; late endosome; lysosome; membrane; plasma membrane; receptor complex; recycling endosome membrane

Molecular Function: glycoprotein binding; identical protein binding; low-density lipoprotein binding; low-density lipoprotein receptor activity; protease binding; protein binding; very-low-density lipoprotein receptor activity

Biological Process: cholesterol absorption; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endocytosis; lipid metabolic process; lipoprotein catabolic process; lipoprotein metabolic process; phospholipid transport; positive regulation of inflammatory response