LDLR / LDL Receptor Recombinant Protein | LDLR recombinant protein

Recombinant Mouse LDLR / LDL Receptor Protein (His tag)

Cat. Num. AAA2547048

• Gene Names: Ldlr; Hlb301
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: LDLR / LDL Receptor; Recombinant Mouse LDLR / LDL Receptor Protein (His tag); Ldlr; Hlb301; LDLR recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 862

• Application Notes: The secreted recombinant mouse LDLR comprises 780 amino acids with a predicted molecular mass of 85.7 kDa. As a result of glycosylation, it migarates as approximately 120-130 kDa protein in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for LDLR recombinant protein

Background: LDL Receptor, also known as LDLR, is a mosaic protein which belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.

Description: A DNA sequence encoding the extracellular domain of mouse LDLR (NP_034830.2) precursor (Met 1-Arg 790) with substitution of Val 23 and Gly 27 by Ala 23 and Cys 27 respectively was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 113195700
• NCBI GeneID: 16835
• NCBI Accession #: NP_034830.2
• NCBI GenBank Nucleotide #: NM_010700.3
• UniProt Accession #: P35951; Q6GTJ9
• Molecular Weight: 94,947 Da
• NCBI Official Full Name: low-density lipoprotein receptor isoform 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: Ldlr
• NCBI Official Synonym Symbols: Hlb301
• NCBI Protein Information: low-density lipoprotein receptor
• UniProt Protein Name: Low-density lipoprotein receptor
• Protein Family: Low-density lipoprotein receptor
• UniProt Gene Name: Ldlr
• UniProt Synonym Gene Names: LDL receptor

Uniprot Description

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 9 A3|9 7.87 cM

Cellular Component: apical part of cell; basolateral plasma membrane; caveola; cell surface; coated pit; early endosome; endosome; external side of plasma membrane; Golgi apparatus; late endosome; lysosome; membrane; plasma membrane; receptor complex; recycling endosome membrane

Molecular Function: glycoprotein binding; identical protein binding; low-density lipoprotein binding; low-density lipoprotein receptor activity; protease binding; protein binding; very-low-density lipoprotein receptor activity

Biological Process: cholesterol absorption; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endocytosis; lipid metabolic process; lipoprotein catabolic process; lipoprotein metabolic process; phospholipid transport; positive regulation of inflammatory response