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Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using MRPS28 antibody at 1:3000 dilution.)

Rabbit anti-Human, Mouse MRPS28 Polyclonal Antibody | anti-MRPS28 antibody

MRPS28 Polyclonal Antibody

Gene Names
MRPS28; MRPS35; HSPC007; MRP-S28; MRP-S35
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
MRPS28; Polyclonal Antibody; MRPS28 Polyclonal Antibody; HSPC007; MRP-S28; MRP-S35; MRPS35; anti-MRPS28 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
MRPS28 polyclonal antibody detects endogenous levels of MRPS28 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
187
Applicable Applications for anti-MRPS28 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IF: 1:20-1:100
Immunogen
Recombinant protein of human MRPS28
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using MRPS28 antibody at 1:3000 dilution.)

Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using MRPS28 antibody at 1:3000 dilution.)
Related Product Information for anti-MRPS28 antibody
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21kDa
NCBI Official Full Name
28S ribosomal protein S28, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein S28
NCBI Official Symbol
MRPS28
NCBI Official Synonym Symbols
MRPS35; HSPC007; MRP-S28; MRP-S35
NCBI Protein Information
28S ribosomal protein S28, mitochondrial
UniProt Protein Name
28S ribosomal protein S28, mitochondrial
Protein Family
UniProt Gene Name
MRPS28
UniProt Synonym Gene Names
MRPS35; MRP-S28; S28mt; MRP-S35; S35mt
UniProt Entry Name
RT28_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS28: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.

Protein type: Mitochondrial; RNA-binding; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 8q21.1-q21.2

Cellular Component: mitochondrial inner membrane; mitochondrial small ribosomal subunit; mitochondrion

Research Articles on MRPS28

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Product Notes

The MRPS28 mrps28 (Catalog #AAA3009922) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRPS28 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MRPS28 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:1000 IF: 1:20-1:100. Researchers should empirically determine the suitability of the MRPS28 mrps28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPS28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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