Mouse LDL Receptor ELISA Kit | LDLR elisa kit

Mouse LDL Receptor ELISA Kit

Cat. Num. AAA8248445

• Gene Names: Ldlr; Hlb301
• Reactivity: Mouse
• Synonyms: LDL Receptor; Mouse LDL Receptor ELISA Kit; Low-density lipoprotein receptor; LDL receptor; LDLR elisa kit

• Reactivity: Mouse
• Specificity: The Mouse LDL Receptor ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse LDL Receptor proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 810

• Samples: Cell Culture Supernates, Serum, Plasma, Urine
• Sensitivity: <12 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDLR elisa kit

Principle of the Assay: The Mouse LDL Receptor ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse LDL Receptor in Cell Culture Supernates, Serum, Plasma, Urine. This assay employs an antibody specific for Mouse LDL Receptor coated on a 96-well plate. Standards and samples are pipetted into the wells and LDL Receptor present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse LDL Receptor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LDL Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: The Low-Density Lipoprotein (LDL) Receptor is a mosaic protein of 839 amino acids (after removal of 21-amino acid signal peptide) that mediates the endocytosis of cholesterol-rich LDL. In humans, the LDL receptor protein is encoded by the LDLR gene. It belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 358030301
• NCBI GeneID: 16835
• NCBI Accession #: NP_001239587.1
• NCBI GenBank Nucleotide #: NM_001252658.1
• UniProt Accession #: P35951; Q6GTJ9
• Molecular Weight: 94,947 Da
• NCBI Official Full Name: low-density lipoprotein receptor isoform 2
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: Ldlr
• NCBI Official Synonym Symbols: Hlb301
• NCBI Protein Information: low-density lipoprotein receptor
• UniProt Protein Name: Low-density lipoprotein receptor
• Protein Family: Low-density lipoprotein receptor
• UniProt Gene Name: Ldlr
• UniProt Synonym Gene Names: LDL receptor
• UniProt Entry Name: LDLR_MOUSE

Uniprot Description

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Cellular Component: apical part of cell; basolateral plasma membrane; caveola; cell surface; coated pit; early endosome; endosome; external side of plasma membrane; Golgi apparatus; late endosome; lysosome; membrane; plasma membrane; receptor complex; recycling endosome membrane

Molecular Function: glycoprotein binding; identical protein binding; low-density lipoprotein binding; low-density lipoprotein receptor activity; protease binding; protein binding; very-low-density lipoprotein receptor activity

Biological Process: cholesterol absorption; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endocytosis; lipid metabolic process; lipoprotein catabolic process; lipoprotein metabolic process; phospholipid transport; positive regulation of inflammatory response