Mouse Interleukin 12B (IL12B) ELISA Kit | IL12B elisa kit

Mouse Interleukin 12B (IL12B) Mini Samples ELISA Kit

Cat. Num. AAA2708215

• Gene Names: Il12b; p40; Il-12b; Il12p40; Il-12p40
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Interleukin 12B (IL12B); Mouse Interleukin 12B (IL12B) Mini Samples ELISA Kit; Interleukin 12B; p40; IL12-B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23; P40 Subunit; IL12B elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 12B (IL12B).; No significant cross-reactivity or interference between Mini Samples Interleukin 12B (IL12B) and analogues was observed.
• Sequence Length: 335

• Applicable Applications for IL12B elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 12B (IL12B) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 12B (IL12B) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 12B (IL12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 12B (IL12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 12B (IL12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Interleukin 12B (IL12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 1170461
• NCBI GeneID: 16160
• UniProt Accession #: P43432
• NCBI Official Full Name: Interleukin-12 subunit beta
• NCBI Official Synonym Full Names: interleukin 12b
• NCBI Official Symbol: Il12b
• NCBI Official Synonym Symbols: p40; Il-12b; Il12p40; Il-12p40
• NCBI Protein Information: interleukin-12 subunit beta
• UniProt Protein Name: Interleukin-12 subunit beta
• Protein Family: Interleukin
• UniProt Gene Name: Il12b
• UniProt Synonym Gene Names: IL-12B; CLMF p40

NCBI Description

This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23. [provided by RefSeq, Dec 2014]

Uniprot Description

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.