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Western Blot (WB) (Western blotAll lanes: ADAT1 antibody at 6ug/mlLane 1: Mouse kidney tissueLane 2: Thp-1 whole cell lysateLane 3: Mouse liver tissueLane 4: MDA-MB-231 whole cell lysateLane 5: HL60 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 56,39 kDaObserved band size: 55 kDa)

Rabbit anti-Human, mouse ADAT1 Polyclonal Antibody | anti-ADAT1 antibody

ADAT1 Antibody

Gene Names
ADAT1; HADAT1
Reactivity
Human, mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
ADAT1; Polyclonal Antibody; ADAT1 Antibody; tRNA-specific adenosine deaminase 1 (EC:3.5.4.34); hADAT1; tRNA-specific adenosine-37 deaminase; anti-ADAT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Liquid
Sequence Length
502
Applicable Applications for anti-ADAT1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human tRNA-specific adenosine deaminase 1 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-10008 / sc-10006
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: ADAT1 antibody at 6ug/mlLane 1: Mouse kidney tissueLane 2: Thp-1 whole cell lysateLane 3: Mouse liver tissueLane 4: MDA-MB-231 whole cell lysateLane 5: HL60 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 56,39 kDaObserved band size: 55 kDa)

Western Blot (WB) (Western blotAll lanes: ADAT1 antibody at 6ug/mlLane 1: Mouse kidney tissueLane 2: Thp-1 whole cell lysateLane 3: Mouse liver tissueLane 4: MDA-MB-231 whole cell lysateLane 5: HL60 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 56,39 kDaObserved band size: 55 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skin tissue using MBS7005549 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human skin tissue using MBS7005549 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7005549 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7005549 at dilution of 1:100)
Related Product Information for anti-ADAT1 antibody
Specifically deaminates adenosine-37 to inosine in tRNA-Ala.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,706 Da
NCBI Official Full Name
tRNA-specific adenosine deaminase 1
NCBI Official Synonym Full Names
adenosine deaminase, tRNA-specific 1
NCBI Official Symbol
ADAT1
NCBI Official Synonym Symbols
HADAT1
NCBI Protein Information
tRNA-specific adenosine deaminase 1
UniProt Protein Name
tRNA-specific adenosine deaminase 1
UniProt Gene Name
ADAT1
UniProt Synonym Gene Names
hADAT1
UniProt Entry Name
ADAT1_HUMAN

NCBI Description

This gene is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, proteins encoded by these genes participate in the pre-mRNA editing of nuclear transcripts. The protein encoded by this gene, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010]

Uniprot Description

ADAT1: Specifically deaminates adenosine-37 to inosine in tRNA- Ala. Belongs to the ADAT1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.5.4.34; RNA processing

Chromosomal Location of Human Ortholog: 16q23.1

Molecular Function: metal ion binding; RNA binding; tRNA-specific adenosine deaminase activity

Biological Process: gene expression; tRNA modification; tRNA processing

Research Articles on ADAT1

Similar Products

Product Notes

The ADAT1 adat1 (Catalog #AAA7005549) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADAT1 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ADAT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the ADAT1 adat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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