Bovine Interleukin 12B (IL12B) ELISA Kit | IL12B elisa kit

Bovine Interleukin 12B (IL12B) High Sensitivity ELISA Kit

Cat. Num. AAA2104746

• Gene Names: IL12B; IL12p40
• Reactivity: Bovine
• Synonyms: Interleukin 12B (IL12B); Bovine Interleukin 12B (IL12B) High Sensitivity ELISA Kit; p40; IL12-B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23; P40 Subunit; IL12B elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL10. No significant cross-reactivity or interference between IL10 and analogues was observed.
• Sequence Length: 327

• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Samples: Simian Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: 6.5pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL12B elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL10 in simian serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL10, biotin-conjugated antibody and enzymeconjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL12B elisa kit

Cytokine; Infection immunity; Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 41386790
• NCBI GeneID: 281857
• NCBI Accession #: NP_776781.1
• NCBI GenBank Nucleotide #: NM_174356.1
• UniProt Accession #: P46282; A9QWR4
• Molecular Weight: 37,050 Da
• NCBI Official Full Name: interleukin-12 subunit beta
• NCBI Official Synonym Full Names: interleukin 12B
• NCBI Official Symbol: IL12B
• NCBI Official Synonym Symbols: IL12p40
• NCBI Protein Information: interleukin-12 subunit beta
• UniProt Protein Name: Interleukin-12 subunit beta
• Protein Family: Interleukin
• UniProt Gene Name: IL12B
• UniProt Synonym Gene Names: IL-12B; CLMF p40

Uniprot Description

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.