Flt3 Ligand Active Protein | FLT3L active protein

Human Flt3 Ligand Protein, His Tag

Cat. Num. AAA9718673

• Gene Names: FLT3LG; FL; FLG3L; FLT3L
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Flt3 Ligand; Human Flt3 Ligand Protein; His Tag; Recombinant Human Flt3 ligand Protein; His Tag (Fms-related tyrosine kinase 3 ligand) expressed in Baculovirus-Insect Cells; FL; FLT3LG; Fms-related tyrosine kinase 3 ligand; FLT3L active protein

• Host: Baculovirus
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
• Sequence: Amino acid sequence derived from human FLT3LG (P49771-1) (Thr27-Pro185) was expressed, with a polyhistidine tag at the N-terminus.
• Sequence Length: 235

• Species: Human
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug of the protein as determined by the LAL method
• Protein Length: The recombinant human human FLT3LG consists of 175 amino acids and predicts a molecular mass of 20.2 KDa. It migrates as an approximately 27 KDa band in SDS-PAGE under reducing conditions.
• Activity: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3. The ED50 for this effect is typically 2-11 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human Flt3 ligand protein, His Tag in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human Flt3 ligand protein, His Tag.)

Related Product Information for FLT3L active protein

FLT3L, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, FLT3L also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 -positive classical DCs and their CD103 -positive tissue counterparts.

NCBI and Uniprot Product Information

• NCBI GI #: 325197197
• NCBI GeneID: 2323
• NCBI Accession #: NP_001191431.1
• NCBI GenBank Nucleotide #: NP_001191431.1
• UniProt Accession #: P49771
• Molecular Weight: 20.2 kDa
• NCBI Official Full Name: fms-related tyrosine kinase 3 ligand isoform 1
• NCBI Official Synonym Full Names: fms related tyrosine kinase 3 ligand
• NCBI Official Symbol: FLT3LG
• NCBI Official Synonym Symbols: FL; FLG3L; FLT3L
• NCBI Protein Information: fms-related tyrosine kinase 3 ligand
• UniProt Protein Name: Fms-related tyrosine kinase 3 ligand
• UniProt Gene Name: FLT3LG
• UniProt Synonym Gene Names: Flt3 ligand; Flt3L
• UniProt Entry Name: FLT3L_HUMAN

NCBI Description

Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]

Uniprot Description

FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Ligand, receptor tyrosine kinase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: extracellular space; membrane; integral to membrane

Molecular Function: protein homodimerization activity; cytokine activity; receptor tyrosine kinase binding; receptor binding

Biological Process: positive regulation of natural killer cell differentiation; regulation of myeloid dendritic cell activation; lymphocyte differentiation; embryonic hemopoiesis; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; signal transduction; positive regulation of myoblast differentiation