Mouse interleukin 11 ELISA Kit | IL11 elisa kit

Mouse Interleukin 11, IL-11 ELISA Kit

Cat. Num. AAA705108

• Gene Names: Il11; IL-11
• Reactivity: Mouse
• Synonyms: interleukin 11; Mouse Interleukin 11; IL-11 ELISA Kit; IL-11 ELISA KIT; AGIF; IL-11; adipogenesis inhibitory factor; oprelvekin; IL11 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse IL11. No significant cross-reactivity or interference between Mouse IL11 and analogues was observed.
• Sequence Length: 199

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 7.8 pg/ml-500 pg/ml
• Sensitivity: 2 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL11 elisa kit

Introduction: Interleukin 11 (IL-11) is a multifunctional cytokine first isolated in 1990 from bone marrow-derived stromal cells. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 is a member of the IL-6-type cytokine family, distinguished based on their use of a common transmembrane signal transducer. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. Besides from lymphopoietic/ hematopoietic and osteotrophic properties, it has functions in many tissues such as brain, gut and testis. IL-11 stimulates the growth of certain lymphocytes and, in the murine model, stimulates an increase in the cortical thickness and strength of long bones.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-11. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-11 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6680393
• NCBI GeneID: 16156
• NCBI Accession #: NP_032376.1
• NCBI GenBank Nucleotide #: NM_008350.4
• UniProt Accession #: P47873
• Molecular Weight: 21,522 Da
• NCBI Official Full Name: interleukin-11 isoform 1
• NCBI Official Synonym Full Names: interleukin 11
• NCBI Official Symbol: Il11
• NCBI Official Synonym Symbols: IL-11
• NCBI Protein Information: interleukin-11
• UniProt Protein Name: Interleukin-11
• Protein Family: Interleukin
• UniProt Gene Name: Il11
• UniProt Synonym Gene Names: IL-11
• UniProt Entry Name: IL11_MOUSE

Uniprot Description

Function: Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production.