Mouse Hyaluronan Synthase 2 (HAS2) ELISA Kit | HAS2 elisa kit

Mouse Hyaluronan Synthase 2 (HAS2) ELISA Kit

Cat. Num. AAA457044

• Reactivity: Mouse
• Synonyms: Hyaluronan Synthase 2 (HAS2); Mouse Hyaluronan Synthase 2 (HAS2) ELISA Kit; Hyaluronate synthase 2; Hyaluronic acid synthase 2; HAS2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of HAS2. No significant cross-reactivity or interference between HAS2 and analogues was observed.
• Sequence Length: 552

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78-5000pg/mL
• Sensitivity: <26pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HAS2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HAS2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HAS2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HAS2 in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HAS2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HAS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HAS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HAS2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 160358868
• NCBI GeneID: 15117
• NCBI Accession #: NP_032242.3
• NCBI GenBank Nucleotide #: NM_008216.3
• UniProt Accession #: P70312; P70411; Q62405; Q68EF7
• Molecular Weight: 63,510 Da
• NCBI Official Full Name: hyaluronan synthase 2
• NCBI Official Synonym Full Names: hyaluronan synthase 2
• NCBI Official Symbol: Has2
• NCBI Protein Information: hyaluronan synthase 2
• UniProt Protein Name: Hyaluronan synthase 2
• Protein Family: Hyaluronan synthase
• UniProt Gene Name: Has2
• UniProt Synonym Gene Names: HA synthase 2

Uniprot Description

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix.