Rabbit anti-Human, Mouse Mapk4 Polyclonal Antibody | anti-Mapk4 antibody

Mapk4, NT (Mapk4, Erk4, Prkm4, Mitogen-activated protein kinase 4, Extracellular signal-regulated kinase 4) (Azide free) (HRP)

Cat. Num. AAA6318840

• Gene Names: Mapk4; Erk3; Prkm4; p63Mapk; A330097D03Rik
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Mapk4; Polyclonal Antibody; NT (Mapk4; Erk4; Prkm4; Mitogen-activated protein kinase 4; Extracellular signal-regulated kinase 4) (Azide free) (HRP); Extracellular signal-regulated kinase 4; anti-Mapk4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Mapk4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Mapk4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of Mouse Mapk4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Mapk4 Antibody (N-term) western blot analysis in C6 cell line lysates (35ug/lane).This demonstrates the Mapk4 antibody detected the Mapk4 protein (arrow).)

Western Blot (WB)

(Mouse Mapk4 Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Mapk4 antibody detected the Mapk4 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Mapk4 Antibody (N-term) (AP14445a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Mapk4 antibody

Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle (By similarity).

Product Categories/Family for anti-Mapk4 antibody

Antibodies; Protein Kinases

References

Klinger, S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(39):16710-16715(2009). Kesavan, C., et al. Bone 41(2):223-230(2007). Kant, S., et al. J. Biol. Chem. 281(46):35511-35519(2006). Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003). Xu, H., et al. J. Biol. Chem. 278(32):30187-30192(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 89337268
• NCBI GeneID: 225724
• NCBI Accession #: NP_766220.2
• NCBI GenBank Nucleotide #: NM_172632.2
• UniProt Accession #: Q6P5G0
• Molecular Weight: 65,574 Da
• NCBI Official Full Name: mitogen-activated protein kinase 4
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 4
• NCBI Official Symbol: Mapk4
• NCBI Official Synonym Symbols: Erk3; Prkm4; p63Mapk; A330097D03Rik
• NCBI Protein Information: mitogen-activated protein kinase 4; ERK-4; MAPK 4; Erk3-related; MAP kinase 4; mitogen activated protein kinase 4; extracellular signal-regulated kinase 4
• UniProt Protein Name: Mitogen-activated protein kinase 4
• UniProt Gene Name: Mapk4
• UniProt Synonym Gene Names: Erk4; Prkm4; MAP kinase 4; MAPK 4; ERK-4
• UniProt Entry Name: MK04_MOUSE