Human Hyaluronan Synthase 2 (HAS2) ELISA Kit | HAS2 elisa kit

Human Hyaluronan Synthase 2 (HAS2) ELISA Kit

Cat. Num. AAA2019490

• Reactivity: Human
• Synonyms: Hyaluronan Synthase 2 (HAS2); Human Hyaluronan Synthase 2 (HAS2) ELISA Kit; Hyaluronate synthase 2; Hyaluronic acid synthase 2; HAS2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hyaluronan Synthase 2 (HAS2).; No significant cross-reactivity or interference between Hyaluronan Synthase 2 (HAS2) and analogues was observed.
• Sequence Length: 552

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyaluronan Synthase 2 (HAS2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyaluronan Synthase 2 (HAS2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HAS2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronan Synthase 2 (HAS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronan Synthase 2 (HAS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronan Synthase 2 (HAS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyaluronan Synthase 2 (HAS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for HAS2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4885391
• NCBI GeneID: 3037
• NCBI Accession #: NP_005319.1
• NCBI GenBank Nucleotide #: NM_005328.2
• UniProt Accession #: Q92819; Q32MM3
• Molecular Weight: 63,566 Da
• NCBI Official Full Name: hyaluronan synthase 2
• NCBI Official Synonym Full Names: hyaluronan synthase 2
• NCBI Official Symbol: HAS2
• NCBI Protein Information: hyaluronan synthase 2
• UniProt Protein Name: Hyaluronan synthase 2
• Protein Family: Hyaluronan synthase
• UniProt Gene Name: HAS2
• UniProt Synonym Gene Names: HA synthase 2
• UniProt Entry Name: HYAS2_HUMAN

NCBI Description

Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1. [provided by RefSeq, Jul 2008]

Uniprot Description

HAS2: Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. Expressed in fibroblasts. Belongs to the nodC/HAS family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis; Transferase; EC 2.4.1.212

Chromosomal Location of Human Ortholog: 8q24.12

Cellular Component: integral to plasma membrane

Molecular Function: hyaluronan synthase activity

Biological Process: extracellular polysaccharide biosynthetic process; hyaluronan biosynthetic process; positive regulation of cell proliferation; kidney development; vasculogenesis; positive regulation of cell migration