Mouse HMG-CoA Reductase (3-hydroxy-3-methylglutaryl-coenzyme A reductase) ELISA Kit | Hmgcr elisa kit

Mouse HMG-CoA Reductase (3-hydroxy-3-methylglutaryl-coenzyme A reductase) ELISA Kit

Cat. Num. AAA7606267

• Gene Names: Hmgcr; Red; HMG-CoAR
• Reactivity: Mouse
• Synonyms: HMG-CoA Reductase (3-hydroxy-3-methylglutaryl-coenzyme A reductase); Mouse HMG-CoA Reductase (3-hydroxy-3-methylglutaryl-coenzyme A reductase) ELISA Kit; Hmgcr elisa kit

• Reactivity: Mouse
• Sequence Length: 887

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Hmgcr elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HMG-CoA Reductase antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HMG-CoA Reductase antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HMG-CoA Reductase amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HMG-CoA Reductase can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 408360266
• NCBI GeneID: 15357
• UniProt Accession #: Q01237; Q5U4I2; G3X8U5
• Molecular Weight: 97,040 Da
• NCBI Official Full Name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase
• NCBI Official Synonym Full Names: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• NCBI Official Symbol: Hmgcr
• NCBI Official Synonym Symbols: Red; HMG-CoAR
• NCBI Protein Information: 3-hydroxy-3-methylglutaryl-coenzyme A reductase
• UniProt Protein Name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase
• UniProt Gene Name: Hmgcr
• UniProt Synonym Gene Names: HMG-CoA reductase

Uniprot Description

HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.

Protein type: EC 1.1.1.34; Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Oxidoreductase; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis

Chromosomal Location of Human Ortholog: 13 D1|13 50.65 cM

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; intracellular membrane-bound organelle; peroxisomal membrane

Molecular Function: coenzyme binding; hydroxymethylglutaryl-CoA reductase (NADPH) activity; hydroxymethylglutaryl-CoA reductase activity; protein homodimerization activity; protein phosphatase 2A binding

Biological Process: cholesterol biosynthetic process; embryonic development; isoprenoid biosynthetic process; negative regulation of apoptosis; negative regulation of MAP kinase activity; positive regulation of cell proliferation; positive regulation of skeletal muscle development; positive regulation of smooth muscle cell proliferation; positive regulation of stress-activated MAPK cascade; protein tetramerization; ubiquinone metabolic process; visual learning