Porcine 3-Hydroxy-3-methylglutaryl CoA reductase ELISA Kit | HMGCR elisa kit

Porcine 3-Hydroxy-3-methylglutaryl CoA reductase ELISA Kit

Cat. Num. AAA743785

• Gene Names: Hmgcr; Red; HMG-CoAR
• Reactivity: Porcine
• Synonyms: 3-Hydroxy-3-methylglutaryl CoA reductase; Porcine 3-Hydroxy-3-methylglutaryl CoA reductase ELISA Kit; HMGCR elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ug/mL.
• Intended Uses: This HMG-CoA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine HMG-CoA. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HMGCR elisa kit

Principle of the Assay: HMG-CoA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HMG-CoA antibody and an HMG-CoA-HRP conjugate. The assay sample and buffer are incubated together with HMG-CoA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMG-CoA concentration since HMG-CoA from samples and HMG-CoA-HRP conjugate compete for the anti-HMG-CoA antibody binding site. Since the number of sites is limited, as more sites are occupied by HMG-CoA from the sample, fewer sites are left to bind HMG-CoA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMG-CoA concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HMGCR elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 160358778
• NCBI GeneID: 15357
• NCBI Accession #: NP_032281.2
• NCBI GenBank Nucleotide #: NM_008255.2
• Molecular Weight: 97,040 Da
• NCBI Official Full Name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase
• NCBI Official Synonym Full Names: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• NCBI Official Symbol: Hmgcr
• NCBI Official Synonym Symbols: Red; HMG-CoAR
• NCBI Protein Information: 3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMG-CoA reductase; 3-hydroxy-3-methylglutaryl-CoA reductase
• UniProt Protein Name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase
• UniProt Gene Name: Hmgcr
• UniProt Synonym Gene Names: HMG-CoA reductase
• UniProt Entry Name: HMDH_MOUSE

Uniprot Description

HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.

Protein type: Membrane protein, integral; EC 1.1.1.34; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Oxidoreductase; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Endoplasmic reticulum

Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane

Molecular Function: hydroxymethylglutaryl-CoA reductase (NADPH) activity; protein homodimerization activity; protein phosphatase 2A binding; hydroxymethylglutaryl-CoA reductase activity; oxidoreductase activity; NADP binding; coenzyme binding; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: steroid metabolic process; negative regulation of MAP kinase activity; cholesterol metabolic process; isoprenoid biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of skeletal muscle development; cholesterol biosynthetic process; positive regulation of stress-activated MAPK cascade; coenzyme A metabolic process; embryonic development; ubiquinone metabolic process; negative regulation of vasodilation; sterol biosynthetic process; positive regulation of cell proliferation; visual learning; lipid metabolic process; steroid biosynthetic process; protein tetramerization; negative regulation of apoptosis