Guinea Pig 3-Hydroxy-3-methylglutaryl CoA reductase ELISA Kit | HMG-CoA elisa kit
Guinea pig 3-Hydroxy-3-methylglutaryl CoA reductase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: HMGCR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HMGCR antibody and an HMGCR-HRP conjugate. The assay sample and buffer are incubated together with HMGCR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMGCR concentration since HMGCR from samples and HMGCR-HRP conjugate compete for the anti-HMGCR antibody binding site. Since the number of sites is limited, as more sites are occupied by HMGCR from the sample, fewer sites are left to bind HMGCR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGCR concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.
Protein type: Oxidoreductase; EC 1.1.1.34; Endoplasmic reticulum; Membrane protein, multi-pass; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Membrane protein, integral; Motility/polarity/chemotaxis
Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane
Molecular Function: hydroxymethylglutaryl-CoA reductase (NADPH) activity; protein homodimerization activity; protein phosphatase 2A binding; hydroxymethylglutaryl-CoA reductase activity; oxidoreductase activity; coenzyme binding; NADP binding; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Biological Process: steroid metabolic process; cholesterol metabolic process; negative regulation of MAP kinase activity; isoprenoid biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of skeletal muscle development; cholesterol biosynthetic process; positive regulation of stress-activated MAPK cascade; coenzyme A metabolic process; embryonic development; ubiquinone metabolic process; negative regulation of vasodilation; sterol biosynthetic process; positive regulation of cell proliferation; visual learning; lipid metabolic process; steroid biosynthetic process; protein tetramerization; negative regulation of apoptosis
