Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase ELISA Kit | HMGCR elisa kit

Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase ELISA Kit

Cat. Num. AAA760848

• Gene Names: HMGCR; LDLCQ3
• Reactivity: Human
• Synonyms: 3-hydroxy-3-methylglutaryl-coenzyme A reductase; Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase ELISA Kit; HMGCR (3-hydroxy-3-methylglutaryl-coenzyme A reductase)/HMG-CoA reductase; HMGCR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HMGCR. No significant cross-reactivity or interference between HMGCR and analogues was observed.
• Sequence Length: 835

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HMGCR were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HMGCR were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HMGCR elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HMGCR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HMGCR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HMGCR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HMGCR can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 196049380
• NCBI GeneID: 3156
• NCBI Accession #: NP_001124468.1
• NCBI GenBank Nucleotide #: NM_001130996.1
• Molecular Weight: 99,752 Da
• NCBI Official Full Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase isoform 2
• NCBI Official Synonym Full Names: 3-hydroxy-3-methylglutaryl-CoA reductase
• NCBI Official Symbol: HMGCR
• NCBI Official Synonym Symbols: LDLCQ3
• NCBI Protein Information: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• UniProt Protein Name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase
• Protein Family: 3-hydroxy-3-methylglutaryl-coenzyme A reductase
• UniProt Gene Name: HMGCR
• UniProt Synonym Gene Names: HMG-CoA reductase
• UniProt Entry Name: HMDH_HUMAN

NCBI Description

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.

Protein type: EC 1.1.1.34; Membrane protein, multi-pass; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Endoplasmic reticulum; Motility/polarity/chemotaxis; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 5q13.3-q14

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; peroxisomal membrane

Molecular Function: coenzyme binding; hydroxymethylglutaryl-CoA reductase (NADPH) activity; hydroxymethylglutaryl-CoA reductase activity; protein binding; protein homodimerization activity; protein phosphatase 2A binding

Biological Process: aging; cellular lipid metabolic process; cholesterol biosynthetic process; coenzyme A metabolic process; isoprenoid biosynthetic process; myoblast differentiation; negative regulation of MAP kinase activity; negative regulation of vasodilation; positive regulation of skeletal muscle development; positive regulation of smooth muscle cell proliferation; positive regulation of stress-activated MAPK cascade; protein tetramerization; response to ethanol; response to nutrient; ubiquinone metabolic process; visual learning