Rabbit CrkL Polyclonal Antibody | anti-CrkL antibody

CrkL (P201) Polyclonal Antibody

Cat. Num. AAA3001552

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CrkL; Polyclonal Antibody; CrkL (P201) Polyclonal Antibody; Crk L; Crk like protein; Crk-like protein; Crkl; Crkol; Oncogene CrkL; anti-CrkL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CrkL (P201) polyclonal antibody detects endogenous levels of CrkL protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 303

• Applicable Applications for anti-CrkL antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 180-230 of Human CrkL.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CrkL (P201) pAb at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of CrkL (P201) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-CrkL antibody

Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa.

NCBI and Uniprot Product Information

• NCBI GI #: 4885153
• NCBI GeneID: 1399
• NCBI Accession #: NP_005198.1
• NCBI GenBank Nucleotide #: NP_005198.1
• UniProt Accession #: P46109
• Molecular Weight: ~ 39kDa
• NCBI Official Full Name: crk-like protein
• NCBI Official Synonym Full Names: CRK like proto-oncogene, adaptor protein
• NCBI Official Symbol: CRKL
• NCBI Protein Information: crk-like protein
• UniProt Protein Name: Crk-like protein
• Protein Family: Crk-like protein
• UniProt Gene Name: CRKL
• UniProt Entry Name: CRKL_HUMAN

NCBI Description

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]

Uniprot Description

CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; endosome

Molecular Function: protein binding; signal transducer activity; SH3/SH2 adaptor activity

Biological Process: anterior/posterior pattern formation; blood vessel development; organ morphogenesis; nerve growth factor receptor signaling pathway; activation of MAPKK activity; thymus development; heart development; Ras protein signal transduction; JNK cascade; parathyroid gland development