Mouse CHI3L3 ELISA Kit | CHI3L3 elisa kit

Mouse CHI3L3 PicoKine ELISA Kit

Cat. Num. AAA1752617

• Gene Names: Chil3; Ym1; ECF-L; Chi3l3; AI505981
• Reactivity: Mouse
• Synonyms: CHI3L3; Mouse CHI3L3 PicoKine ELISA Kit; Mouse CHI3L3; chitinase-like 3; Chitinase-like protein 3; Beta-N-acetylhexosaminidase Ym1; Chitinase-3-like protein 3; ECF-L; Eosinophil chemotactic cytokine; Secreted protein Ym1; Chil3; Chi3l3; Ym1; CHI3L3 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Chil3
• Sequence Length: 398

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 312pg/ml-20000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHI3L3 elisa kit

Principle of the Assay: The Picokine™ Mouse Chil3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Chil3 with a 96-well strip plate that is pre-coated with antibody specific for Chil3. The detection antibody is a biotinylated antibody specific for Chil3. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Chil3 with immunogen: Expression system for standard: NSO, Immunogen sequence: Y22-Y398. The kit is analytically validated with ready to use reagents. To measure Mouse Chil3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Chil3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Chil3 in the sample.!!Background/Introduction: Chitinase 3-like 3, also known as ECF-L and Ym1, is a secreted 377 amino acid lectin that binds heparin and GlcN oligomers. This gene is mapped to 3 F2.2; 3 46.49 Cm. CHI3L3 is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins, but lacks enzymatic activity.

NCBI and Uniprot Product Information

• NCBI GI #: 254281348
• NCBI GeneID: 12655
• NCBI Accession #: NP_034022.2
• NCBI GenBank Nucleotide #: NP_034022.2
• UniProt Accession #: O35744
• NCBI Official Full Name: chitinase-like protein 3
• NCBI Official Synonym Full Names: chitinase-like 3
• NCBI Official Symbol: Chil3
• NCBI Official Synonym Symbols: Ym1; ECF-L; Chi3l3; AI505981
• NCBI Protein Information: chitinase-like protein 3
• UniProt Protein Name: Chitinase-like protein 3
• UniProt Gene Name: Chil3
• UniProt Synonym Gene Names: Chi3l3; Ym1
• UniProt Entry Name: CHIL3_MOUSE

NCBI Description

The protein encoded by this gene is similar to bacterial chitinases but lacks chitinase activity. The encoded protein is thought to function as a lectin and may be involved in inflammation and allergy. [provided by RefSeq, Sep 2015]

Uniprot Description

CHI3L3: Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger. Binds chitin and heparin. Has no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Protein type: EC 3.2.1.52

Cellular Component: cytoplasm; cytoplasmic membrane-bound vesicle; endoplasmic reticulum; extracellular region; nucleus

Molecular Function: beta-N-acetylhexosaminidase activity; carbohydrate binding; chitin binding; chitinase activity; hydrolase activity; hydrolase activity, acting on glycosyl bonds; hydrolase activity, hydrolyzing O-glycosyl compounds

Biological Process: carbohydrate metabolic process; chitin catabolic process; inflammatory response; metabolic process; polysaccharide catabolic process