Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin) , member 5 ELISA Kit | SERPINA5 elisa kit

Human Plasma serine protease inhibitor, SERPINA5 ELISA Kit

Cat. Num. AAA938556

• Reactivity: Human
• Synonyms: serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 5; Human Plasma serine protease inhibitor; SERPINA5 ELISA Kit; Human Plasma serine protease inhibitor (SERPINA5) ELISA kit; PAI3; PCI; PLANH3; PROCI; plasminogen activator inhibitor III; plasminogen activator inhibitor-3; serine (or cysteine) proteinase inhibitor; SERPINA5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human SERPINA5. No significant cross-reactivity or interference between human SERPINA5 and analogues was observed.
• Sequence Length: 404

• Samples: Serum, plasma, tissue homogenates, cell culture supernates and urine
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: <0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SERPINA5 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 28603766
• NCBI GeneID: 338050
• NCBI Accession #: NP_788819.1
• NCBI GenBank Nucleotide #: NM_176646.3
• UniProt Accession #: Q9N2I2; A7E3W0
• Molecular Weight: 45,297 Da
• NCBI Official Full Name: plasma serine protease inhibitor
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
• NCBI Official Symbol: SERPINA5
• NCBI Protein Information: plasma serine protease inhibitor; PCI; serpin A5; protein C inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
• UniProt Protein Name: Plasma serine protease inhibitor
• Protein Family: Plasma serine protease inhibitor
• UniProt Gene Name: SERPINA5
• UniProt Synonym Gene Names: PCI
• UniProt Entry Name: IPSP_BOVIN

Uniprot Description

Function: Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and proinflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary-type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue-and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as a hydrophobic hormone carrier by its binding to retinoic acid 2 PublicationsManual assertion based on experiment in:Ref.1

Enzyme regulation: Its inhibitory activity is greatly enhanced in the presence of glycosaminoglycans, heparin, thrombomodulin and phospholipids vesicles