Mouse Galectin 3 ELISA Kit | LGALS3 elisa kit

Mouse Galectin 3 ELISA Kit

Cat. Num. AAA824630

• Gene Names: Lgals3; GBP; L-34; gal3; Mac-2
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Galectin 3; Mouse Galectin 3 ELISA Kit; MAC2; Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen; LGALS3 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse Galectin 3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Galectin 3 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 264

• Applicable Applications for LGALS3 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma
• Sensitivity: <10 pg/mL
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LGALS3 elisa kit

Principle of the Assay: MBS Mouse Galectin 3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Galectin 3 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse Galectin 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Galectin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Galectin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 126679
• NCBI GeneID: 16854
• UniProt Accession #: P16110
• Molecular Weight: 27,515 Da
• NCBI Official Full Name: Galectin-3
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 3
• NCBI Official Symbol: Lgals3
• NCBI Official Synonym Symbols: GBP; L-34; gal3; Mac-2
• NCBI Protein Information: galectin-3; gal-3; CBP 35; lectin L-29; 35 kDa lectin; mac-2 antigen; igE-binding protein; laminin-binding protein; galactose-specific lectin 3; L-34 galactoside-binding lectin; carbohydrate-binding protein 35
• UniProt Protein Name: Galectin-3
• UniProt Gene Name: Lgals3
• UniProt Synonym Gene Names: Gal-3; CBP 35
• UniProt Entry Name: LEG3_MOUSE

Uniprot Description

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Motility/polarity/chemotaxis; Cell surface; Extracellular matrix

Cellular Component: spliceosome; extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; membrane; mitochondrial inner membrane; cytoplasm; extracellular region; immunological synapse; nucleus; external side of plasma membrane

Molecular Function: monosaccharide binding; protein binding; advanced glycation end-product receptor activity; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity; disaccharide binding

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; immune system process; positive regulation of serotonin secretion; negative regulation of endocytosis; RNA splicing; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; signal transduction; macrophage chemotaxis; monocyte chemotaxis; positive regulation of angiogenesis; positive chemotaxis; positive regulation of cell proliferation; eosinophil chemotaxis; innate immune response; mRNA processing; cell differentiation; skeletal development; negative regulation of apoptosis