Mouse lectin, galactoside-binding, soluble, 3 ELISA Kit | LGALS3 elisa kit

Mouse Galectin 3, GAL-3 ELISA Kit

Cat. Num. AAA702906

• Gene Names: Lgals3; GBP; L-34; gal3; Mac-2
• Reactivity: Mouse
• Synonyms: lectin; galactoside-binding; soluble; 3; Mouse Galectin 3; GAL-3 ELISA Kit; Mouse Galectin 3 (GAL-3) ELISA Kit; CBP35; GAL3; GALBP; GALIG; LGALS2; MAC2; IgE-binding protein; MAC-2 antigen; carbohydrate-binding protein 35; galactose-specific lectin 3; galectin 3; galectin-3 internal; laminin-binding protein; LGALS3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse LGALS3. No significant cross-reactivity or interference between Mouse LGALS3 and analogues was observed.
• Sequence Length: 264

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Developmental Protein
• Protein Biological Process 3: Differentiation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LGALS3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GAL-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAL-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAL-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAL-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126679
• NCBI GeneID: 16854
• UniProt Accession #: P16110
• Molecular Weight: 27,515 Da
• NCBI Official Full Name: Galectin-3
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 3
• NCBI Official Symbol: Lgals3
• NCBI Official Synonym Symbols: GBP; L-34; gal3; Mac-2
• NCBI Protein Information: galectin-3; gal-3; CBP 35; lectin L-29; 35 kDa lectin; mac-2 antigen; igE-binding protein; laminin-binding protein; galactose-specific lectin 3; L-34 galactoside-binding lectin; carbohydrate-binding protein 35
• UniProt Protein Name: Galectin-3
• UniProt Gene Name: Lgals3
• UniProt Synonym Gene Names: Gal-3; CBP 35
• UniProt Entry Name: LEG3_MOUSE

Uniprot Description

Function: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells 1 PublicationManual assertion based on experiment in:Ref.9