Mouse Fibronectin type III domain-containing protein 5, FNDC5/FRCP2 ELISA Kit | FNDC5/FRCP2 elisa kit

Mouse Fibronectin type III domain-containing protein 5, FNDC5/FRCP2 ELISA Kit

Cat. Num. AAA166291

• Gene Names: FNDC5; FRCP2; irisin
• Reactivity: Mouse
• Synonyms: Fibronectin type III domain-containing protein 5; FNDC5/FRCP2; Mouse Fibronectin type III domain-containing protein 5; FNDC5/FRCP2 ELISA Kit; FNDC5/FRCP2 elisa kit

• Reactivity: Mouse
• Sequence Length: 212

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.5ng/ml - 200ng/ml
• Sensitivity: 0.24ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FNDC5/FRCP2 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Fibronectin Type III Domain-containing p-Protein 5 (also known as FNDC5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse FNDC5 antibody. FNDC5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse FNDC5 Antibody is added and binds to FNDC5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FNDC5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse FNDC5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 511094000
• NCBI GeneID: 252995
• NCBI Accession #: NP_715637.2
• NCBI GenBank Nucleotide #: NM_153756.2
• UniProt Accession #: Q8NAU1; Q6P6D9; Q7Z676; A6NMC9; D3DPQ6
• Molecular Weight: 20,281 Da
• NCBI Official Full Name: fibronectin type III domain-containing protein 5 isoform 2 preproprotein
• NCBI Official Synonym Full Names: fibronectin type III domain containing 5
• NCBI Official Symbol: FNDC5
• NCBI Official Synonym Symbols: FRCP2; irisin
• NCBI Protein Information: fibronectin type III domain-containing protein 5
• UniProt Protein Name: Fibronectin type III domain-containing protein 5
• UniProt Gene Name: FNDC5
• UniProt Synonym Gene Names: FRCP2

NCBI Description

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.