Rabbit FNDC5 Polyclonal Antibody | anti-FNDC5 antibody

FNDC5 Rabbit Polyclonal

Cat. Num. AAA769410

• Gene Names: FNDC5; FRCP2; irisin
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Purity: >=95% as determined by SDS-PAGE; Purification: Immunogen Affinity Purified
• Synonyms: FNDC5; Polyclonal Antibody; FNDC5 Rabbit Polyclonal; FRCP2; Irisin; anti-FNDC5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Please decide the specificity by homology
• Purity/Purification: Purity: >=95% as determined by SDS-PAGE; Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3,
• Sequence Length: 212

• Applicable Applications for anti-FNDC5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB : 1:200-1:2000; IP :1:200-1:100; IHC : 1:20-1:200
• Immunogen: Fibronectin type III domain containing 5
• Preparation and Storage: Store at -20°C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skeletal muscle slide using MBS769410 (FNDC5 Antibody) at dilution of 1:50)

Western Blot (WB)

(Mouse placenta tissue were subjected to SDS PAGE followed by western blot with MBS769410 (FNDC5 antibody) at dilution of 1:1000)

Immunoprecipitation (IP)

(IP Result of anti-FNDC5 (IP: MBS769410, 3ug; Detection: MBS769410 1:300) with mouse liver tissue lysate 4000ug.)

Related Product Information for anti-FNDC5 antibody

Background: Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.

NCBI and Uniprot Product Information

• NCBI GI #: 511094000
• NCBI GeneID: 252995
• NCBI Accession #: NP_715637.2
• NCBI GenBank Nucleotide #: NM_153756.2
• UniProt Accession #: Q8NAU1; Q6P6D9; Q7Z676; A6NMC9; D3DPQ6
• Molecular Weight: 20 kDa
• NCBI Official Full Name: fibronectin type III domain-containing protein 5 isoform 2 preproprotein
• NCBI Official Synonym Full Names: fibronectin type III domain containing 5
• NCBI Official Symbol: FNDC5
• NCBI Official Synonym Symbols: FRCP2; irisin
• NCBI Protein Information: fibronectin type III domain-containing protein 5
• UniProt Protein Name: Fibronectin type III domain-containing protein 5
• Protein Family: Fibronectin type III domain-containing protein
• UniProt Gene Name: FNDC5
• UniProt Synonym Gene Names: FRCP2
• UniProt Entry Name: FNDC5_HUMAN

NCBI Description

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: endoplasmic reticulum; extracellular region; integral to membrane; peroxisomal membrane; plasma membrane

Molecular Function: hormone activity

Biological Process: response to muscle activity