Human Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit | FNDC5 elisa kit

Human Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit

Cat. Num. AAA452539

• Gene Names: FNDC5; FRCP2; irisin
• Reactivity: Human
• Synonyms: Fibronectin Type III Domain Containing Protein 5 (FNDC5); Human Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit; FRCP2; Irisin; •Fibronectin type III repeat-containing protein 2; FNDC5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FNDC5. No significant cross-reactivity or interference between FNDC5 and analogues was observed.
• Sequence Length: 181

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.63ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FNDC5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FNDC5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FNDC5 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FNDC5 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FNDC5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FNDC5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FNDC5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FNDC5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 511093995
• NCBI GeneID: 252995
• NCBI Accession #: NP_001165411.2
• NCBI GenBank Nucleotide #: NM_001171940.1
• UniProt Accession #: Q8NAU1; Q6P6D9; Q7Z676; A6NMC9; D3DPQ6
• Molecular Weight: 15,527 Da
• NCBI Official Full Name: fibronectin type III domain-containing protein 5 isoform 3 preproprotein
• NCBI Official Synonym Full Names: fibronectin type III domain containing 5
• NCBI Official Symbol: FNDC5
• NCBI Official Synonym Symbols: FRCP2; irisin
• NCBI Protein Information: fibronectin type III domain-containing protein 5
• UniProt Protein Name: Fibronectin type III domain-containing protein 5
• Protein Family: Fibronectin type III domain-containing protein
• UniProt Gene Name: FNDC5
• UniProt Synonym Gene Names: FRCP2

NCBI Description

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.