Human Orexin A ELISA Kit | Orexin A elisa kit

Human Orexin A ELISA Kit

Cat. Num. AAA701515

• Gene Names: HCRT; OX; PPOX; NRCLP1
• Reactivity: Human
• Synonyms: Orexin A; Human Orexin A ELISA Kit; NRCLP1; OX; PPOX; orexin; HCRT; hypocretin (orexin) neuropeptide precursor; Orexin A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human orexin A. No significant cross-reactivity or interference between human orexin A and analogues was observed.
• Sequence Length: 131

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: < 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Orexin A elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for orexin A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any orexin A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for orexin A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of orexin A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4557635
• NCBI GeneID: 3060
• NCBI Accession #: NP_001515.1
• NCBI GenBank Nucleotide #: NM_001524.1
• UniProt Accession #: O43612
• Molecular Weight: 13,363 Da
• NCBI Official Full Name: orexin
• NCBI Official Synonym Full Names: hypocretin (orexin) neuropeptide precursor
• NCBI Official Symbol: HCRT
• NCBI Official Synonym Symbols: OX; PPOX; NRCLP1
• NCBI Protein Information: orexin; prepro-orexin
• UniProt Protein Name: Orexin
• UniProt Gene Name: HCRT
• UniProt Synonym Gene Names: OX; PPORX; PPOX; Hcrt; Hcrt1; Hcrt2
• UniProt Entry Name: OREX_HUMAN

NCBI Description

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010]

Uniprot Description

Function: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.