Human fibronectin type III domain containing 5 ELISA Kit | FNDC5 elisa kit

Human Fibronectin type III domain-containing protein 5, FNDC5 ELISA Kit

Cat. Num. AAA925506

• Gene Names: FNDC5; FRCP2; irisin
• Reactivity: Human
• Synonyms: fibronectin type III domain containing 5; Human Fibronectin type III domain-containing protein 5; FNDC5 ELISA Kit; Human Fibronectin type III domain-containing protein 5 (FNDC5) ELISA kit; FRCP2; ; FNDC5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human FNDC5. No significant cross-reactivity or interference between human FNDC5 and analogues was observed.
• Sequence Length: 181

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 7.8 ng/ml -500 ng/ml
• Sensitivity: Typically less than 0.95 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FNDC5 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FNDC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FNDC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FNDC5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FNDC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 511093995
• NCBI GeneID: 252995
• NCBI Accession #: NP_001165411.2
• NCBI GenBank Nucleotide #: NM_001171940.1
• UniProt Accession #: Q8NAU1; Q6P6D9; Q7Z676; A6NMC9; D3DPQ6
• Molecular Weight: 23,659 Da
• NCBI Official Full Name: fibronectin type III domain-containing protein 5 isoform 3 preproprotein
• NCBI Official Synonym Full Names: fibronectin type III domain containing 5
• NCBI Official Symbol: FNDC5
• NCBI Official Synonym Symbols: FRCP2; irisin
• NCBI Protein Information: fibronectin type III domain-containing protein 5; fibronectin type III repeat-containing protein 2
• UniProt Protein Name: Fibronectin type III domain-containing protein 5
• Protein Family: Fibronectin type III domain-containing protein
• UniProt Gene Name: FNDC5
• UniProt Synonym Gene Names: FRCP2
• UniProt Entry Name: FNDC5_HUMAN

NCBI Description

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: peroxisomal membrane; endoplasmic reticulum; integral to membrane; extracellular region; plasma membrane

Molecular Function: hormone activity

Biological Process: response to muscle activity