Mouse Fatty Acid Binding Protein 5, Epidermal (FABP5) ELISA Kit | FABP5 elisa kit

Mouse Fatty Acid Binding Protein 5, Epidermal (FABP5) ELISA Kit

Cat. Num. AAA454015

• Gene Names: Fabp5; Klbp; mal1; Fabpe; E-FABP; PA-FABP
• Reactivity: Mouse
• Synonyms: Fatty Acid Binding Protein 5; Epidermal (FABP5); Mouse Fatty Acid Binding Protein 5; Epidermal (FABP5) ELISA Kit; E-FABP; EFABP; PA-FABP; PAFABP; Psoriasis-Associated; Epidermal-type fatty acid-binding protein; Psoriasis-associated fatty acid-binding protein homolog; FABP5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FABP5. No significant cross-reactivity or interference between FABP5 and analogues was observed.
• Sequence Length: 135

• Samples: Tissue homogenates, breast milk and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FABP5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FABP5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FABP5 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FABP5 in mouse tissue homogenates, breast milk and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FABP5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FABP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FABP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FABP5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6754450
• NCBI GeneID: 16592
• NCBI Accession #: NP_034764.1
• NCBI GenBank Nucleotide #: NM_010634.3
• UniProt Accession #: Q05816
• Molecular Weight: 15,137 Da
• NCBI Official Full Name: fatty acid-binding protein, epidermal isoform 1
• NCBI Official Synonym Full Names: fatty acid binding protein 5, epidermal
• NCBI Official Symbol: Fabp5
• NCBI Official Synonym Symbols: Klbp; mal1; Fabpe; E-FABP; PA-FABP
• NCBI Protein Information: fatty acid-binding protein, epidermal; uncharacterized protein LOC16592
• UniProt Protein Name: Fatty acid-binding protein, epidermal
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: Fabp5
• UniProt Synonym Gene Names: Fabpe; Klbp; Mal1; E-FABP; PA-FABP

NCBI Description

The protein encoded by this gene is part of the fatty acid binding protein family (FABP). FABPs are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands and participate in fatty acid uptake, transport, and metabolism. In humans this gene has been associated with psoriasis and type 2 diabetes. In mouse deficiency of this gene in combination with a deficiency in Fabp4 confers protection against atherosclerosis, diet-induced obesity, insulin resistance and experimental autoimmune encephalomyelitis (the mouse model for multiple sclerosis). Alternative splicing results in multiple transcript variants that encode different protein isoforms. The mouse genome contains many pseudogenes similar to this locus. [provided by RefSeq, Jan 2013]

Uniprot Description

High specificity for fatty acids. Highest affinity for C18 chain length ().