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Western Blot (WB) (Western blot analysis of PDCL3 in human brain tissue lysate with PDCL3 antibody at 1 μg/ml.)

Rabbit PDCL3 Polyclonal Antibody | anti-PDCL3 antibody

PDCL3 Antibody

Gene Names
PDCL3; VIAF; PHLP3; VIAF1; HTPHLP; PHLP2A
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PDCL3 antibody is affinity chromatography purified via peptide column.
Synonyms
PDCL3; Polyclonal Antibody; PDCL3 Antibody; VIAF; PHLP3; VIAF1; HTPHLP; PHLP2A; PhLP2A; Phosducin-like protein 3; VIAF-1; phosducin-like 3; anti-PDCL3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PDCL3 antibody is human, mouse and rat reactive. PDCL3 antibody is predicted to not cross-react with other members of the PDCL protein family.
Purity/Purification
PDCL3 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
239
Applicable Applications for anti-PDCL3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
PDCL3 antibody can be used for detection of PDCL by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry at 5 mug/mL. For Immunoflorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
PDCL3 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human PDCL3.
Buffer
PDCL3 antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
PDCL3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of PDCL3 in human brain tissue lysate with PDCL3 antibody at 1 μg/ml.)

Western Blot (WB) (Western blot analysis of PDCL3 in human brain tissue lysate with PDCL3 antibody at 1 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of PDCL3 in mouse lver tissue with PDCL3 antibody at 5 μg/mL.)

Immunohistochemistry (IHC) (Immunohistochemistry of PDCL3 in mouse lver tissue with PDCL3 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PDCL3 in mouse liver tissue with PDCL3 antibody at 20 μg/mL.)

Immunofluorescence (IF) (Immunofluorescence of PDCL3 in mouse liver tissue with PDCL3 antibody at 20 μg/mL.)
Related Product Information for anti-PDCL3 antibody
Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1,2). PDCL3 is highly homologous to PDCL and shares an N-terminal helix domain and a C-terminal thioredoxin-fold (Trx-fold) domain (3). Along with the related protein PDCL2, PDCL3 interacts with the chaperonin CCT and modulates CCT-mediated actin and tubulin folding (4). Modulation of PDCL3 levels by MAPK phosphorylation and RhoA-dependent changes also promote cytoskeletal remodeling (5).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 26 kDa

Observed: 25 kDa
NCBI Official Full Name
phosducin-like protein 3
NCBI Official Synonym Full Names
phosducin-like 3
NCBI Official Symbol
PDCL3
NCBI Official Synonym Symbols
VIAF; PHLP3; VIAF1; HTPHLP; PHLP2A
NCBI Protein Information
phosducin-like protein 3; phPL3; VIAF-1; IAP-associated factor VIAF1; viral IAP-associated factor 1
UniProt Protein Name
Phosducin-like protein 3
Protein Family
UniProt Gene Name
PDCL3
UniProt Synonym Gene Names
PhLP2A; VIAF1; VIAF-1
UniProt Entry Name
PDCL3_HUMAN

NCBI Description

This gene encodes a member of the phosducin-like protein family and is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin. Members of the phosducin-like protein family have been shown to bind to the beta-gamma subunits of G proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

PDCL3: Modulates the activation of caspases during apoptosis. Is a substrate for Orgyia pseudotsugata multicapsid polyhedrosis virus (OpMNPV) IAP-mediated ubiquitination. Belongs to the phosducin family.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: cytoplasm

Molecular Function: protein binding; vascular endothelial growth factor receptor 2 binding

Biological Process: positive regulation of angiogenesis; protein folding; viral reproduction; apoptosis; positive regulation of endothelial cell proliferation; angiogenesis; regulation of peptidyl-tyrosine phosphorylation

Research Articles on PDCL3

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Product Notes

The PDCL3 pdcl3 (Catalog #AAA153675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDCL3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDCL3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). PDCL3 antibody can be used for detection of PDCL by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry at 5 mug/mL. For Immunoflorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the PDCL3 pdcl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDCL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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