Human FABP5 ELISA Kit | FABP5 elisa kit

Human FABP5 (Fatty Acid Binding Protein 5, Epidermal) ELISA Kit

Cat. Num. AAA2501304

• Gene Names: FABP5; EFABP; KFABP; E-FABP; PAFABP; PA-FABP
• Reactivity: Human
• Synonyms: FABP5; Human FABP5 (Fatty Acid Binding Protein 5; Epidermal) ELISA Kit; FABP5 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human FABP5 in samples. No significant cross-reactivity or interference between Human FABP5 and analogues was observed.
• Sequence Length: 135

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.31-20ng/mL
• Sensitivity: 0.19ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human FABP5 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human FABP5 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FABP5 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human FABP5 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FABP5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP5 and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human FABP5. You can calculate the concentration of Human FABP5 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557581
• NCBI GeneID: 2171
• NCBI Accession #: NP_001435.1
• NCBI GenBank Nucleotide #: NM_001444.2
• UniProt Accession #: Q01469; B2R4K0
• Molecular Weight: 15,164 Da
• NCBI Official Full Name: fatty acid-binding protein, epidermal
• NCBI Official Synonym Full Names: fatty acid binding protein 5 (psoriasis-associated)
• NCBI Official Symbol: FABP5
• NCBI Official Synonym Symbols: EFABP; KFABP; E-FABP; PAFABP; PA-FABP
• NCBI Protein Information: fatty acid-binding protein, epidermal; epidermal-type fatty acid-binding protein; psoriasis-associated fatty acid-binding protein homolog
• UniProt Protein Name: Fatty acid-binding protein, epidermal
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP5
• UniProt Synonym Gene Names: E-FABP; PA-FABP
• UniProt Entry Name: FABP5_HUMAN

NCBI Description

This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may play roles in fatty acid uptake, transport, and metabolism. Polymorphisms in this gene are associated with type 2 diabetes. The human genome contains many pseudogenes similar to this locus.[provided by RefSeq, Feb 2011]

Uniprot Description

FABP5: High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 8q21.13

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: protein binding; transporter activity; lipid binding; fatty acid binding

Biological Process: epidermis development; phosphatidylcholine biosynthetic process; glucose metabolic process; lipid metabolic process; glucose transport