Mouse Alpha Internexin/ NF-66 Monoclonal Antibody | anti-INA antibody

Anti-Alpha-Internexin (NF66)

Cat. Num. AAA500002

• Gene Names: Ina; Nf66; Inexa; Intlaa
• Reactivity: Most mammalian
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Total IgG fraction
• Synonyms: Alpha Internexin/ NF-66; Monoclonal Antibody; Anti-Alpha-Internexin (NF66); anti-INA antibody

• Host: Mouse
• Reactivity: Most mammalian
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1D2
• Specificity: Specific for the ~66k alpha Internexin protein. Can be used on formalinfixed cells in tissue culture, cryostat sections, and Western blotting. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections.
• Purity/Purification: Total IgG fraction
• Form/Format: Liquid containing 10mM sodium azide.
• Sequence Length: 505

• Applicable Applications for anti-INA antibody: Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:2,000; IHC: 1:1,000; IF: 1:250
• Antigen: Recombinant rat alpha-internexin expressed in and purified from E. coli.
• Immunogen Information: Recombinant rat alpha-internexin expressed in and purified from E. coli
• Immunogen Species: Rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in a wide variety of mammalian species.
• Biological Significance: Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-INA antibody

Mouse monoclonal antibody

References

• Pachter, J and Liem, RKH. Alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. J Cell Biol 101:1316-22 (1985).
• McGraw et al. Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons. J Neurosci 22:4955-63 (2002).
• Cairns NJ et al. alpha-Internexin aggregates are abundant in neuronal intermediate filament inclusion disease (NIFID) but rare in other neurodegenerative diseases. Acta Neuropathol (Berl). May 28 [Epub ahead of print] (2004).
• Cairns NJ et al. alpha-internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. Am J Pathol. 164:2153-61 (2004).
• Rajasalu T, Teesalu K, Janmey PA, Uibo R. Demonstration of natural autoantibodies against the neurofilament protein alpha-internexin in sera of patients with endocrine autoimmunity and healthy individuals. Immunol Lett. 94:153-60 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 9506811
• NCBI GeneID: 24503
• NCBI Accession #: NP_062001.1
• NCBI GenBank Nucleotide #: NM_019128.4
• UniProt Accession #: P23565
• Molecular Weight: 66
• NCBI Official Full Name: alpha-internexin
• NCBI Official Synonym Full Names: internexin neuronal intermediate filament protein, alpha
• NCBI Official Symbol: Ina
• NCBI Official Synonym Symbols: Nf66; Inexa; Intlaa
• NCBI Protein Information: alpha-internexin; alpha-Inx; internexin, alpha
• UniProt Protein Name: Alpha-internexin
• Protein Family: Alpha-internexin
• UniProt Gene Name: Ina
• UniProt Synonym Gene Names: Inexa; Alpha-Inx
• UniProt Entry Name: AINX_RAT

NCBI Description

may play a role in neurogenesis; may be involved in neuronal regeneration in response to injury [RGD, Feb 2006]

Uniprot Description

Function: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges

By similarity.

Developmental stage: Levels of this protein reach a maximum at embryonic day 16 and decline into adulthood.

Post-translational modification: O-glycosylated

By similarity.

Sequence similarities: Belongs to the intermediate filament family.