Human Anoctamin 6 (ANO6) ELISA Kit | ANO6 elisa kit

Human Anoctamin 6 (ANO6) ELISA Kit

Cat. Num. AAA453455

• Gene Names: ANO6; SCTS; BDPLT7; TMEM16F
• Reactivity: Human
• Synonyms: Anoctamin 6 (ANO6); Human Anoctamin 6 (ANO6) ELISA Kit; Primary response gene B94 protein; TNF alpha-induced protein 2; TNFAIP2; ANO6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ANO6. No significant cross-reactivity or interference between ANO6 and analogues was observed.
• Sequence Length: 910

• Samples: Human tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.127ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ANO6 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ANO6 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANO6 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ANO6 in human tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANO6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ANO6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ANO6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ANO6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 218156299
• NCBI GeneID: 196527
• NCBI Accession #: NP_001020527.2
• NCBI GenBank Nucleotide #: NM_001025356.2
• UniProt Accession #: Q4KMQ2; Q8N3Q2; A6NNM6; B9EGG0; E7ENK4; E9PB30; E9PCT2
• Molecular Weight: 108,102 Da
• NCBI Official Full Name: anoctamin-6 isoform a
• NCBI Official Synonym Full Names: anoctamin 6
• NCBI Official Symbol: ANO6
• NCBI Official Synonym Symbols: SCTS; BDPLT7; TMEM16F
• NCBI Protein Information: anoctamin-6
• UniProt Protein Name: Anoctamin-6
• Protein Family: Anoctamin
• UniProt Gene Name: ANO6
• UniProt Synonym Gene Names: TMEM16F; SCAN channel

NCBI Description

This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets and osteoblasts. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (). Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.