Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Epithelial neutrophil activating peptide 78 (ENA-78) Standard Curve.)
Mouse Epithelial Neutrophil Activating Peptide 78 (ENA-78) ELISA Kit | ENA-78 elisa kit
Mouse Epithelial Neutrophil Activating Peptide 78 (ENA-78) ELISA Kit
Gene Names
Cxcl5; LIX; Cxcl6; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
Reactivity
Mouse
Synonyms
Epithelial Neutrophil Activating Peptide 78 (ENA-78); Mouse Epithelial Neutrophil Activating Peptide 78 (ENA-78) ELISA Kit; CXCL5; ENA-78; SCYB5; epithelial-derived neutrophil activating protein 78; epithelial-derived neutrophil-activating peptide 78; neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; small ind; ENA-78 elisa kit
Reactivity
Mouse
Specificity
Mouse Epithelial neutrophil activating peptide 78 (ENA-78) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CXCL5. No significant cross-reactivity or interference between Mouse CXCL5 and analogues was observed.
Sequence Length
132
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Epithelial neutrophil activating peptide 78 (ENA-78) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Epithelial neutrophil activating peptide 78 (ENA-78) Standard Curve.)
Related Product Information for ENA-78 elisa kit
Description: This Mouse Epithelial neutrophil activating peptide 78 (ENA-78) ELISA Kit employs a two-site sandwich ELISA to quantitate CXCL5 in samples. An antibody specific for CXCL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ENA-78 is a CXC chemokine that was originally isolated from media conditioned by the growth of a mouse lung type-II alveolar epithelial cell line (A549) stimulated by IL-1 or TNF. The full-length cDNA encodes a 114 amino acid (aa) residue precursor protein with a 36 aa residue signal peptide that is cleaved to generate the 78 aa residue secreted protein. ENA-78 shares significant amino acid sequence identity with NAP-2 (53%), GRO,, and (52%, 48% and 51%, respectively), and IL-8 (22%). The gene for ENA-78 has been mapped to chromosome 4q13-q21. Like other CXC chemokines, ENA-78 is a neutrophil attractant and activator in vitro. Based on cross-desensitization experiments, it has been suggested that ENA-78 activity can be mediated through the IL-8 receptor system.
Background: ENA-78 is a CXC chemokine that was originally isolated from media conditioned by the growth of a mouse lung type-II alveolar epithelial cell line (A549) stimulated by IL-1 or TNF. The full-length cDNA encodes a 114 amino acid (aa) residue precursor protein with a 36 aa residue signal peptide that is cleaved to generate the 78 aa residue secreted protein. ENA-78 shares significant amino acid sequence identity with NAP-2 (53%), GRO,, and (52%, 48% and 51%, respectively), and IL-8 (22%). The gene for ENA-78 has been mapped to chromosome 4q13-q21. Like other CXC chemokines, ENA-78 is a neutrophil attractant and activator in vitro. Based on cross-desensitization experiments, it has been suggested that ENA-78 activity can be mediated through the IL-8 receptor system.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
C-X-C motif chemokine 5
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 5
NCBI Official Symbol
Cxcl5
NCBI Official Synonym Symbols
LIX; Cxcl6; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
NCBI Protein Information
C-X-C motif chemokine 5
UniProt Protein Name
C-X-C motif chemokine 5
UniProt Gene Name
Cxcl5
UniProt Synonym Gene Names
Scyb5
UniProt Entry Name
CXCL5_MOUSE
NCBI Description
This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils and to have homeostatic and inflammatory functions. In mouse, deficiency of this gene is associated with increased lung inflammation that is neutrophil-dependent. [provided by RefSeq, May 2013]
Uniprot Description
CXCL6: Chemotactic for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Cellular Component: extracellular space
Molecular Function: chemokine activity; CXCR chemokine receptor binding
Biological Process: cytokine production; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; leukocyte homeostasis; organ regeneration; positive regulation of JAK-STAT cascade; positive regulation of leukocyte chemotaxis; regulation of cell proliferation; regulation of chemokine production; response to lipopolysaccharide
Research Articles on ENA-78
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Product Notes
The Mouse ENA-78 cxcl5 (Catalog #AAA9718165) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718165 ELISA Kit recognizes Mouse ENA-78. It is sometimes possible for the material contained within the vial of "Epithelial Neutrophil Activating Peptide 78 (ENA-78), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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