Rabbit anti-Human, Mouse SNAI1 Polyclonal Antibody | anti-SNAI1 antibody

SNAI1, NT (R8) (Zinc Finger Protein SNAI1, Protein Snail Homolog 1, Protein sna, SNAI1, SNAH (FITC)

Cat. Num. AAA6499955

• Gene Names: SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SNAI1; Polyclonal Antibody; NT (R8) (Zinc Finger Protein SNAI1; Protein Snail Homolog 1; Protein sna; SNAH (FITC); anti-SNAI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SNAI1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SNAI1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human SNAI1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of anti-SNAI1 Antibody (N-term R8) in mouse lung tissue lysates (35ug/lane). SNAI1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of SNAI1(arrow) using rabbit polyclonal SNAI1 Antibody (N-term R8). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SNAI1 gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-SNAI1 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Deloukas, P., et al., Nature 414(6866):865-871 (2001). 3. Batlle, E., et al., Nat. Cell Biol. 2(2):84-89 (2000). 4. Paznekas, W.A., et al., Genomics 62(1):42-49 (1999). 5. Twigg, S.R., et al., Hum. Genet. 105(4):320-326 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 18765741
• NCBI GeneID: 6615
• NCBI Accession #: NP_005976
• NCBI GenBank Nucleotide #: NM_005985.3
• UniProt Accession #: O95863; Q9P113; Q9UBP7; Q9UHH7; B2R842
• Molecular Weight: 29,083 Da
• NCBI Official Full Name: zinc finger protein SNAI1
• NCBI Official Synonym Full Names: snail family transcriptional repressor 1
• NCBI Official Symbol: SNAI1
• NCBI Official Synonym Symbols: SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• NCBI Protein Information: zinc finger protein SNAI1
• UniProt Protein Name: Zinc finger protein SNAI1
• Protein Family: Protein snail
• UniProt Gene Name: SNAI1
• UniProt Synonym Gene Names: SNAH; Protein sna
• UniProt Entry Name: SNAI1_HUMAN

NCBI Description

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

Snail1: a protein of the C2H2-type zinc-finger family that regulates transcription. Involved in embryonic mesoderm formation. Plays a role in the epithelial to mesenchymal transition (EMT). EMT is characterized by decreased levels of the epithelial markers E-cadherin and alpha- and beta-catenin, and increased cellular migration and expression of the mesenchymal markers fibronectin, vimentin, N-cadherin and smooth muscle alpha-actin.E Snail binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. NF-kappaB binds and regulates the snail promoter and is critical for EMT, and inhibition of NF-kappaB activity lowered snail levels and morphologically reversed the EMT.

Protein type: Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; kinase binding

Biological Process: osteoblast differentiation; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell migration; hair follicle morphogenesis; mesoderm formation; positive regulation of transcription, DNA-dependent; epithelial to mesenchymal transition; negative regulation of transcription from RNA polymerase II promoter; palate development; positive regulation of cell migration