Mouse CXCL5/ENA-78 ELISA Kit | CXCL5 elisa kit

Mouse CXCL5/ENA-78 PicoKine ELISA Kit

Cat. Num. AAA176511

• Gene Names: Cxcl5; LIX; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
• Reactivity: Mouse
• Synonyms: CXCL5/ENA-78; Mouse CXCL5/ENA-78 PicoKine ELISA Kit; C-X-C motif chemokine 5; chemokine (C-X-C motif) ligand 5; AMCF II; AMCFII; C X C motif chemokine 5; Chemokine (C X C motif) ligand 5; CXCL 5; ENA 78; ENA78; Epithelial derived neutrophil activating protein 78; GCP 2; LIX; Neutrophil activating peptide ENA 78; Neutrophil activating protein 78; SCYB5; Scyb6; Small inducible cytokine B5; Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78); Small inducible cytokine subfamily B; member 15; CXCL5 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CXCL5
• Sequence Length: 132

• Samples: Cell culture supernates, serum and plasma(heparin1 EDTA, citrate}.
• Assay Type: Quantitative Sandwich
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse CXCL5/ENA-78 PicoKine ELISA Kit standard curve )

Related Product Information for CXCL5 elisa kit

Principle of the Assay: The mouse CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL5 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: V45-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL5 amount of sample captured in plate.

Background/Introduction: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-y. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

NCBI and Uniprot Product Information

• NCBI GI #: 408360041
• NCBI GeneID: 20311
• UniProt Accession #: P50228; Q8K3I3
• Molecular Weight: 14,235 Da
• NCBI Official Full Name: C-X-C motif chemokine 5
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 5
• NCBI Official Symbol: Cxcl5
• NCBI Official Synonym Symbols: LIX; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
• NCBI Protein Information: C-X-C motif chemokine 5; cytokine LIX; small-inducible cytokine B5; granulocyte chemotactic protein-2; small inducible cytokine B subfamily, member 5; small inducible cytokine subfamily B, member 15
• UniProt Protein Name: C-X-C motif chemokine 5
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: Cxcl5
• UniProt Synonym Gene Names: Scyb5
• UniProt Entry Name: CXCL5_MOUSE

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils and to have homeostatic and inflammatory functions. In mouse, deficiency of this gene is associated with increased lung inflammation that is neutrophil-dependent. [provided by RefSeq, May 2013]

Uniprot Description

CXCL6: Chemotactic for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding; cytokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; organ regeneration; positive regulation of JAK-STAT cascade; cytokine production; response to lipopolysaccharide; immune response; positive regulation of leukocyte chemotaxis; inflammatory response; chemotaxis; regulation of chemokine production; leukocyte homeostasis; regulation of cell proliferation