Mouse Endoribonuclease Dicer (DICER1) ELISA Kit | DICER1 elisa kit

Mouse Endoribonuclease Dicer (DICER1) ELISA Kit

Cat. Num. AAA9717888

• Gene Names: Dicer1; D12Ertd7e; mKIAA0928; 1110006F08Rik
• Reactivity: Mouse
• Synonyms: Endoribonuclease Dicer (DICER1); Mouse Endoribonuclease Dicer (DICER1) ELISA Kit; DICER1; DCR1; Dicer; HERNA; KIAA0928; Dicer1; Dcr-1 homolog; K12H4.8-LIKE; dicer 1; double-stranded RNA-specific endoribonuclease; dicer1; helicase with RNAse motif; helicase-moi; DICER1 elisa kit

• Reactivity: Mouse
• Sequence Length: 1906

• Assay Type: Sandwich
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for DICER1 elisa kit

Principle of the Assay: Mouse Endoribonuclease Dicer (DICER1) ELISA Kit 48T employs a two-site sandwich ELISA to quantitate DICER1 in samples. An antibody specific for DICER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DICER1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Mouse DICER1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of DICER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background/Introduction: Dicer, also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. Being part of the RNase III family, Dicer cleaves double-stranded RNA (dsRNA) and pre-microRNA (pre-miRNA) into short double-stranded RNA fragments called small interfering RNA and microRNA, respectively. These fragments are approximately 20-25 base pairs long with a two-base overhang on the 3' ends. Dicer facilitates the activation of the RNA-induced silencing complex (RISC), which is essential for RNA interference. RISC has a catalytic component Argonaute, which is an endonuclease capable of degrading messenger RNA (mRNA).

NCBI and Uniprot Product Information

• NCBI GI #: 117168271
• NCBI GeneID: 192119
• NCBI Accession #: NP_683750.2
• NCBI GenBank Nucleotide #: NP_683750.2
• UniProt Accession #: Q8R418
• NCBI Official Full Name: endoribonuclease Dicer
• NCBI Official Synonym Full Names: dicer 1, ribonuclease type III
• NCBI Official Symbol: Dicer1
• NCBI Official Synonym Symbols: D12Ertd7e; mKIAA0928; 1110006F08Rik
• NCBI Protein Information: endoribonuclease Dicer
• UniProt Protein Name: Endoribonuclease Dicer
• Protein Family: Endoribonuclease
• UniProt Gene Name: Dicer1
• UniProt Synonym Gene Names: Dicer; Mdcr
• UniProt Entry Name: DICER_MOUSE

Uniprot Description

DICER1: a cytoplasmic type-III RNase that plays a central role in RNA interference (RNAi) pathways to produce the active small RNAs that represses gene expression. Possesses a DEAD box RNA helicase motif in its amino terminus and a dsRNA-binding motif in the carboxy terminus. Dicer, along with Ago2 andTRBP, are the main components of the RNA-induced silencing complex (RISC). Fragile X syndrome repeats form RNA hairpins that are cut by Dicer but do not activate the interferon-inducible protein kinase, PKR. Four alternatively spliced isoforms have been described.

Protein type: EC 3.1.26.3; Helicase; Cell development/differentiation; Ribonuclease; RNA-binding; RNA processing

Cellular Component: growth cone; axon; dendrite; cytoplasm; ER-Golgi intermediate compartment; nucleus

Molecular Function: miRNA binding; nuclease activity; hydrolase activity; RNA binding; metal ion binding; nucleotide binding; deoxyribonuclease I activity; endoribonuclease activity, producing 5'-phosphomonoesters; helicase activity; endoribonuclease activity; protein binding; ribonuclease III activity; nucleic acid binding; endonuclease activity; double-stranded RNA binding; ATP binding

Biological Process: regulation of myelination; regulation of neuron differentiation; regulation of cell cycle; anatomical structure development; rRNA catabolic process; miRNA-mediated gene silencing, miRNA loading onto RISC; post-embryonic development; RNA processing; hair follicle development; regulation of Notch signaling pathway; pre-microRNA processing; RNA-mediated gene silencing; defense response to virus; meiotic spindle organization and biogenesis; embryonic limb morphogenesis; regulation of odontogenesis of dentine-containing teeth; spleen development; mRNA stabilization; RNA interference, targeting of mRNA for destruction; RNA interference, conversion of ds siRNA to ss siRNA; miRNA-mediated gene silencing, production of miRNAs; branching morphogenesis of a tube; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; RNA interference, production of siRNA; myoblast cell differentiation involved in skeletal muscle regeneration; zygote asymmetric cell division; regulation of oligodendrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; olfactory bulb interneuron differentiation; cardiac muscle cell development; regulation of neurogenesis; regulation of cell differentiation; epidermis morphogenesis; angiogenesis; DNA fragmentation during apoptosis; regulation of viral genome replication; positive regulation of myelination; regulation of protein amino acid phosphorylation; positive regulation of Schwann cell differentiation; hair follicle morphogenesis; inner ear receptor cell development; multicellular organism growth; stem cell maintenance; spindle assembly; spinal cord motor neuron differentiation; cell proliferation; myelin formation in the peripheral nervous system; gut development; regulation of RNA metabolic process; cartilage development; nerve development; reproductive structure development; neurite morphogenesis; lung development