Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Endoribonuclease Dicer (DICER1) Standard Curve.)
Human Endoribonuclease Dicer (DICER1) ELISA Kit | DICER1 elisa kit
Human Endoribonuclease Dicer (DICER1) ELISA Kit
Gene Names
DICER1; DCR1; GLOW; MNG1; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE
Reactivity
Human
Synonyms
Endoribonuclease Dicer (DICER1); Human Endoribonuclease Dicer (DICER1) ELISA Kit; DICER1; DCR1; Dicer; HERNA; KIAA0928; Dicer1; Dcr-1 homolog; K12H4.8-LIKE; dicer 1; double-stranded RNA-specific endoribonuclease; dicer1; helicase with RNAse motif; helicase-moi; DICER1 elisa kit
Reactivity
Human
Specificity
Human Endoribonuclease Dicer (DICER1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DICER1. No significant cross-reactivity or interference between Human DICER1 and analogues was observed.
Sequence Length
1829
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Endoribonuclease Dicer (DICER1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Endoribonuclease Dicer (DICER1) Standard Curve.)
Related Product Information for DICER1 elisa kit
Description: This Human Endoribonuclease Dicer (DICER1) ELISA Kit employs a two-site sandwich ELISA to quantitate DICER1 in samples. An antibody specific for DICER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DICER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DICER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DICER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DICER1 encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants.
Background: DICER1 encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
endoribonuclease Dicer isoform 2
NCBI Official Synonym Full Names
dicer 1, ribonuclease III
NCBI Official Symbol
DICER1
NCBI Official Synonym Symbols
DCR1; GLOW; MNG1; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE
NCBI Protein Information
endoribonuclease Dicer
UniProt Protein Name
Endoribonuclease Dicer
Protein Family
UniProt Gene Name
DICER1
UniProt Synonym Gene Names
DICER; HERNA; KIAA0928; Helicase MOI
UniProt Entry Name
DICER_HUMAN
NCBI Description
This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]
Research Articles on DICER1
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Product Notes
The Human DICER1 dicer1 (Catalog #AAA9715721) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715721 ELISA Kit recognizes Human DICER1. It is sometimes possible for the material contained within the vial of "Endoribonuclease Dicer (DICER1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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