Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.)
Mouse Dicer Monoclonal Antibody | anti-Dicer1 antibody
Dicer Antibody: ATTO 390
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.)
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.)
Scientific Background: Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)
NCBI and Uniprot Product Information
Uniprot Description
DICER1: a cytoplasmic type-III RNase that plays a central role in RNA interference (RNAi) pathways to produce the active small RNAs that represses gene expression. Possesses a DEAD box RNA helicase motif in its amino terminus and a dsRNA-binding motif in the carboxy terminus. Dicer, along with Ago2 andTRBP, are the main components of the RNA-induced silencing complex (RISC). Fragile X syndrome repeats form RNA hairpins that are cut by Dicer but do not activate the interferon-inducible protein kinase, PKR. Four alternatively spliced isoforms have been described.
Protein type: RNA processing; Helicase; Cell development/differentiation; EC 3.1.26.3; Ribonuclease; RNA-binding
Cellular Component: growth cone; axon; cytoplasm; dendrite; ER-Golgi intermediate compartment; nucleus
Molecular Function: miRNA binding; nuclease activity; hydrolase activity; RNA binding; metal ion binding; nucleotide binding; deoxyribonuclease I activity; helicase activity; endoribonuclease activity, producing 5'-phosphomonoesters; endoribonuclease activity; protein binding; ribonuclease III activity; nucleic acid binding; endonuclease activity; double-stranded RNA binding; ATP binding
Biological Process: regulation of myelination; regulation of cell cycle; regulation of neuron differentiation; anatomical structure development; rRNA catabolic process; miRNA-mediated gene silencing, miRNA loading onto RISC; post-embryonic development; RNA processing; regulation of Notch signaling pathway; hair follicle development; pre-microRNA processing; RNA-mediated gene silencing; defense response to virus; meiotic spindle organization and biogenesis; regulation of odontogenesis of dentine-containing teeth; embryonic limb morphogenesis; spleen development; RNA interference, targeting of mRNA for destruction; mRNA stabilization; RNA interference, conversion of ds siRNA to ss siRNA; miRNA-mediated gene silencing, production of miRNAs; branching morphogenesis of a tube; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; RNA interference, production of siRNA; myoblast cell differentiation involved in skeletal muscle regeneration; zygote asymmetric cell division; regulation of oligodendrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; olfactory bulb interneuron differentiation; cardiac muscle cell development; regulation of neurogenesis; epidermis morphogenesis; regulation of cell differentiation; DNA fragmentation during apoptosis; angiogenesis; regulation of viral genome replication; positive regulation of myelination; positive regulation of Schwann cell differentiation; regulation of protein amino acid phosphorylation; hair follicle morphogenesis; inner ear receptor cell development; multicellular organism growth; stem cell maintenance; spindle assembly; spinal cord motor neuron differentiation; cell proliferation; myelin formation in the peripheral nervous system; gut development; cartilage development; regulation of RNA metabolic process; nerve development; reproductive structure development; neurite morphogenesis; lung development
Research Articles on Dicer1
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Product Notes
The Dicer1 dicer1 (Catalog #AAA800430) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Dicer Antibody: ATTO 390 reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Dicer can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). 1ug/ml WB. Researchers should empirically determine the suitability of the Dicer1 dicer1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dicer, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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