Mouse Dipeptidyl Peptidase 8 (DPP8) ELISA Kit | DPP8 elisa kit

Mouse Dipeptidyl Peptidase 8 (DPP8) ELISA Kit

Cat. Num. AAA453921

• Gene Names: Dpp8; AI666706; DPP VIII; 2310004I03Rik; 4932434F09Rik
• Reactivity: Mouse
• Synonyms: Dipeptidyl Peptidase 8 (DPP8); Mouse Dipeptidyl Peptidase 8 (DPP8) ELISA Kit; DP8; DPRP1; MSTP141; MSTP097; MSTP135; dipeptidylpeptidase 8; Dipeptidyl Peptidase IV-Related Protein-1; Prolyl Dipeptidase DPP VIII; DPP8 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of DPP8. No significant cross-reactivity or interference between DPP8 and analogues was observed.
• Sequence Length: 892

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.113ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DPP8 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DPP8 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPP8 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DPP8 in mouse tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DPP8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DPP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DPP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DPP8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31542571
• NCBI GeneID: 74388
• NCBI Accession #: NP_083182.2
• NCBI GenBank Nucleotide #: NM_028906.2
• UniProt Accession #: Q80YA7; Q9D4G6
• Molecular Weight: 102,186 Da
• NCBI Official Full Name: dipeptidyl peptidase 8
• NCBI Official Synonym Full Names: dipeptidylpeptidase 8
• NCBI Official Symbol: Dpp8
• NCBI Official Synonym Symbols: AI666706; DPP VIII; 2310004I03Rik; 4932434F09Rik
• NCBI Protein Information: dipeptidyl peptidase 8
• UniProt Protein Name: Dipeptidyl peptidase 8
• Protein Family: Dipeptidyl peptidase
• UniProt Gene Name: Dpp8
• UniProt Synonym Gene Names: DP8; DPP VIII

Uniprot Description

Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. May play a role in T-cell activation and immune function ().