Mouse Dipeptidyl Peptidase 8 (DPP8) ELISA Kit | DPP8 elisa kit

Mouse Dipeptidyl Peptidase 8 (DPP8) ELISA Kit

Cat. Num. AAA2705295

• Gene Names: DPP8; DP8; DPRP1; DPRP-1; MST097; MSTP097; MSTP135; MSTP141
• Reactivity: Mouse
• Synonyms: Dipeptidyl Peptidase 8 (DPP8); Mouse Dipeptidyl Peptidase 8 (DPP8) ELISA Kit; DP8; DPRP1; MSTP141; MSTP097; MSTP135; dipeptidylpeptidase 8; Dipeptidyl Peptidase IV-Related Protein-1; Prolyl Dipeptidase DPP VIII; DPP8 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dipeptidyl Peptidase 8 (DPP8).; No significant cross-reactivity or interference between Dipeptidyl Peptidase 8 (DPP8) and analogues was observed.
• Sequence Length: 725

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dipeptidyl Peptidase 8 (DPP8) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dipeptidyl Peptidase 8 (DPP8) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPP8 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase 8 (DPP8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidyl Peptidase 8 (DPP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase 8 (DPP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipeptidyl Peptidase 8 (DPP8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37577091
• NCBI GeneID: 54878
• NCBI Accession #: NP_932065.1
• NCBI GenBank Nucleotide #: NM_197961.3
• UniProt Accession #: Q6V1X1; Q7Z4C8; Q7Z4D3; Q7Z4E1; Q8IWG7; Q8NEM5; Q96JX1; Q9HBM2; Q9HBM3; Q9HBM4; Q9HBM5; Q9NXF4
• Molecular Weight: 83,398 Da
• NCBI Official Full Name: dipeptidyl peptidase 8 isoform 4
• NCBI Official Synonym Full Names: dipeptidyl peptidase 8
• NCBI Official Symbol: DPP8
• NCBI Official Synonym Symbols: DP8; DPRP1; DPRP-1; MST097; MSTP097; MSTP135; MSTP141
• NCBI Protein Information: dipeptidyl peptidase 8
• UniProt Protein Name: Dipeptidyl peptidase 8
• Protein Family: Dipeptidyl peptidase
• UniProt Gene Name: DPP8
• UniProt Synonym Gene Names: DPRP1; DP8; DPRP-1; DPP VIII

NCBI Description

This gene encodes a member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. May play a role in T-cell activation and immune function.