Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Diacylglycerol kinase epsilon (DGKE) Standard Curve.)
Human Diacylglycerol Kinase epsilon (DGKE) ELISA Kit | DGKE elisa kit
Human Diacylglycerol Kinase epsilon (DGKE) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Diacylglycerol kinase epsilon (DGKE) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Diacylglycerol kinase epsilon (DGKE) Standard Curve.)
Background: Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.
NCBI and Uniprot Product Information
NCBI Description
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008]
Uniprot Description
DGKE: Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition. Belongs to the eukaryotic diacylglycerol kinase family.
Protein type: Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; Membrane protein, multi-pass; Membrane protein, integral; EC 2.7.1.107; Motility/polarity/chemotaxis; Kinase, lipid
Chromosomal Location of Human Ortholog: 17q22
Cellular Component: membrane; cytoplasm; integral to membrane; plasma membrane
Molecular Function: metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity
Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation; phospholipid biosynthetic process
Disease: Nephrotic Syndrome, Type 7
Research Articles on DGKE
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Product Notes
The Human DGKE dgke (Catalog #AAA9715743) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715743 ELISA Kit recognizes Human DGKE. It is sometimes possible for the material contained within the vial of "Diacylglycerol Kinase epsilon (DGKE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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