Mouse Diacylglycerol Kinase Alpha (DGKa) ELISA Kit | DGKa elisa kit

Mouse Diacylglycerol Kinase Alpha (DGKa)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2125266

• Gene Names: Dgka; 80kDa; Dagk1; AW146112
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Diacylglycerol Kinase Alpha (DGKa); Mouse Diacylglycerol Kinase Alpha (DGKa)(Sandwich ELISA) ELISA Kit; ELISA Kit for Diacylglycerol Kinase Alpha (DGKa); DGKa/Diacylglycerol Kinase Alpha; DGK-A; DAGK; DAGK1; DGK-alpha; 80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGKa elisa kit

• Reactivity: Mouse
• Specificity: Reactive with Mouse Diacylglycerol Kinase Alpha (DGKa); This assay has high sensitivity and excellent specificity for detection of Diacylglycerol Kinase Alpha (DGKa). No significant cross-reactivity or interference between Diacylglycerol Kinase Alpha (DGKa) and analogues was observed.

• Applicable Applications for DGKa elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• Detection Method: Double-antibody Sandwich
• Assay Length: 3 hours
• Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diacylglycerol Kinase Alpha (DGKa) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diacylglycerol Kinase Alpha (DGKa) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100.; Intra-Assay: CV<10%.; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Dehydrogenase 1 (GLUD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Dehydrogenase 1 (GLUD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Dehydrogenase 1 (GLUD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutamate Dehydrogenase 1 (GLUD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 50ul Stop Solution. Read at 450nm immediately.
• Preparation and Storage: Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life); The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for DGKa elisa kit

Enzyme & Kinase; Endocrinology; Neuro science

NCBI and Uniprot Product Information

• NCBI GI #: 31560474
• NCBI GeneID: 13139
• NCBI Accession #: NP_058091.2
• NCBI GenBank Nucleotide #: NM_016811.2
• UniProt Accession #: O88673; Q922X2
• Molecular Weight: 82,762 Da
• NCBI Official Full Name: diacylglycerol kinase alpha
• NCBI Official Synonym Full Names: diacylglycerol kinase, alpha
• NCBI Official Symbol: Dgka
• NCBI Official Synonym Symbols: 80kDa; Dagk1; AW146112
• NCBI Protein Information: diacylglycerol kinase alpha; DGK-alpha; DAG kinase alpha; diglyceride kinase alpha; 80 kDa diacylglycerol kinase; diacylglycerol kinase alpha Flt2-Pr2; truncated diacylglycerol kinase alpha Flt1-Pr1; truncated diacylglycerol kinase alpha Flt1-Pr2
• UniProt Protein Name: Diacylglycerol kinase alpha
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: Dgka
• UniProt Synonym Gene Names: Dagk1; DAG kinase alpha; DGK-alpha
• UniProt Entry Name: DGKA_MOUSE