Typical Testing Data/Standard Curve (for reference only)
Mouse Interleukin 27 Receptor Alpha (IL27Ra) ELISA Kit | IL27Ra elisa kit
Mouse Interleukin 27 Receptor Alpha (IL27Ra)(Sandwich ELISA) ELISA Kit
Gene Names
Il27ra; CRL1; Tccr; Wsx1; WSX-1; IL-27R; zcytor1
Reactivity
Mouse
Applications
ELISA
Synonyms
Interleukin 27 Receptor Alpha (IL27Ra); Mouse Interleukin 27 Receptor Alpha (IL27Ra)(Sandwich ELISA) ELISA Kit; ELISA Kit for Interleukin 27 Receptor Alpha (IL27Ra); IL27Ra/Interleukin 27 Receptor Alpha; CRL1; IL27R; TCCR; WSX1; Zcytor1; T-Cell Cytokine Receptor Type 1; Cytokine receptor-like 1; Type I T-cell cytokine receptor; IL27Ra elisa kit
Reactivity
Mouse
Specificity
Reactive with Mouse Interleukin 27 Receptor Alpha (IL27Ra)
This assay has high sensitivity and excellent specificity for detection of Interleukin 27 Receptor Alpha (IL27Ra). No significant cross-reactivity or interference between Interleukin 27 Receptor Alpha (IL27Ra) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Interleukin 27 Receptor Alpha (IL27Ra). No significant cross-reactivity or interference between Interleukin 27 Receptor Alpha (IL27Ra) and analogues was observed.
Applicable Applications for IL27Ra elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Assay Type
Sandwich ELISA
Sample Types
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.057ng/mL
Detection Method
Double-antibody Sandwich
Assay Length
3 hours
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 27 Receptor Alpha (IL27Ra) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 27 Receptor Alpha (IL27Ra) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 27 Receptor Alpha (IL27Ra) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pentosidine (PTD) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pentosidine (PTD) and unlabeled Pentosidine (PTD)(Standards or samples) with the pre-coated antibody specific to Pentosidine (PTD). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Pentosidine (PTD) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Pentosidine (PTD) in the sample.
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
Preparation and Storage
Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for IL27Ra elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,093 Da
NCBI Official Full Name
interleukin-27 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 27 receptor, alpha
NCBI Official Symbol
Il27ra
NCBI Official Synonym Symbols
CRL1; Tccr; Wsx1; WSX-1; IL-27R; zcytor1
NCBI Protein Information
interleukin-27 receptor subunit alpha; IL-27RA; IL-27R-alpha; IL-27R subunit alpha; T cell cytokine receptor; IL-27 receptor subunit alpha; type I T-cell cytokine receptor; cytokine receptor family, class 1 (WSXWS), member 1; T cell cytokine receptor; cyt
UniProt Protein Name
Interleukin-27 receptor subunit alpha
Protein Family
UniProt Gene Name
Il27ra
UniProt Synonym Gene Names
Tccr; Wsx1; IL-27 receptor subunit alpha; IL-27R subunit alpha; IL-27R-alpha; IL-27RA; TCCR
UniProt Entry Name
I27RA_MOUSE
Uniprot Description
IL27RA: Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1- type immune responses. Also appears to be involved in innate defense mechanisms. Belongs to the type I cytokine receptor family. Type 2 subfamily.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane
Molecular Function: protein binding; interleukin-27 receptor activity
Biological Process: positive regulation of interferon-gamma production; defense response to Gram-positive bacterium; negative regulation of T-helper 2 type immune response; positive regulation of T-helper 1 type immune response; regulation of isotype switching to IgG isotypes
Research Articles on IL27Ra
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Product Notes
The Mouse IL27Ra il27ra (Catalog #AAA2125234) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2125234 ELISA Kit recognizes Mouse IL27Ra. AAA Biotech's Interleukin 27 Receptor Alpha (IL27Ra) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the IL27Ra il27ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 27 Receptor Alpha (IL27Ra), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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