Rat Adenosine Deaminase (ADA) ELISA Kit | ADA elisa kit

Rat Adenosine Deaminase (ADA)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2125242

• Reactivity: Rat
• Applications: ELISA
• Synonyms: Adenosine Deaminase (ADA); Rat Adenosine Deaminase (ADA)(Sandwich ELISA) ELISA Kit; ELISA Kit for Adenosine Deaminase (ADA); ADA/Adenosine Deaminase; Adenosine Aminohydrolase; ADA elisa kit

• Reactivity: Rat
• Specificity: Reactive with Rat Adenosine Deaminase (ADA); This assay has high sensitivity and excellent specificity for detection of Adenosine Deaminase (ADA). No significant cross-reactivity or interference between Adenosine Deaminase (ADA) and analogues was observed.

• Applicable Applications for ADA elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA
• Sample Types: serum, plasma, tissue homogenatesand other biological fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.53ng/mL
• Detection Method: Double-antibody Sandwich
• Assay Length: 3 hours
• Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosine Deaminase (ADA) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosine Deaminase (ADA) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100.; Intra-Assay: CV<10%.; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Linker For Activation Of T-Cell (LAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Linker For Activation Of T-Cell (LAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Linker For Activation Of T-Cell (LAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Linker For Activation Of T-Cell (LAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 50ul Stop Solution. Read at 450nm immediately.
• Preparation and Storage: Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life); The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for ADA elisa kit

Immune molecule