Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Deoxyribonuclease-1 (DNASE1) Standard Curve.)
Mouse Deoxyribonuclease-1 (DNASE1) ELISA Kit | DNASE1 elisa kit
Mouse Deoxyribonuclease-1 (DNASE1) ELISA Kit
Gene Names
Dnase1; Dnl1; DNaseI; AI788650
Reactivity
Mouse
Synonyms
Deoxyribonuclease-1 (DNASE1); Mouse Deoxyribonuclease-1 (DNASE1) ELISA Kit; DNASE1; DKFZp686H0155; DNL1; DRNI; FLJ38093; FLJ44902; DNase I; lysosomal; Dornase alfa; human urine deoxyribonuclease I; DNASE1 elisa kit
Reactivity
Mouse
Specificity
Mouse Deoxyribonuclease-1 (DNASE1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DNASE1. No significant cross-reactivity or interference between Mouse DNASE1 and analogues was observed.
Sequence Length
284
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Deoxyribonuclease-1 (DNASE1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Deoxyribonuclease-1 (DNASE1) Standard Curve.)
Related Product Information for DNASE1 elisa kit
Description: This Mouse Deoxyribonuclease-1 (DNASE1) ELISA Kit employs a two-site sandwich ELISA to quantitate DNASE1 in samples. An antibody specific for DNASE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNASE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNASE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNASE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. It has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.DNase I binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. The function of this interaction is unclear. However since actin-bound DNase I is enzymatically inactive, the DNase-actin complex might be a storage form of DNase I that prevents damage of the genetic information.
Background: DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. It has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.DNase I binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. The function of this interaction is unclear. However since actin-bound DNase I is enzymatically inactive, the DNase-actin complex might be a storage form of DNase I that prevents damage of the genetic information.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
deoxyribonuclease-1
NCBI Official Synonym Full Names
deoxyribonuclease I
NCBI Official Symbol
Dnase1
NCBI Official Synonym Symbols
Dnl1; DNaseI; AI788650
NCBI Protein Information
deoxyribonuclease-1
UniProt Protein Name
Deoxyribonuclease-1
Protein Family
UniProt Gene Name
Dnase1
UniProt Synonym Gene Names
Dnl1; DNase I
Uniprot Description
DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family.
Protein type: Deoxyribonuclease; EC 3.1.21.1; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 16 A1|16 2.37 cM
Cellular Component: extracellular region; nucleus
Molecular Function: actin binding; deoxyribonuclease activity; deoxyribonuclease I activity; endonuclease activity; hydrolase activity; nuclease activity
Biological Process: apoptosis; DNA catabolic process
Research Articles on DNASE1
Similar Products
Product Notes
The Mouse DNASE1 dnase1 (Catalog #AAA9717836) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717836 ELISA Kit recognizes Mouse DNASE1. It is sometimes possible for the material contained within the vial of "Deoxyribonuclease-1 (DNASE1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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