DNASE1 / Deoxyribonuclease I / DNL1 Recombinant Protein | DNL1 recombinant protein

Recombinant Human DNASE1 / Deoxyribonuclease I / DNL1 Protein (His tag)

Cat. Num. AAA2545245

• Gene Names: DNASE1; DNL1; DRNI
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: DNASE1 / Deoxyribonuclease I / DNL1; Recombinant Human DNASE1 / Deoxyribonuclease I / DNL1 Protein (His tag); DNASE1; DNL1; DRNI; DNL1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 282

• Application Notes: The recombinant human DNASE1 consists of 271 amino acids and predicts a molecular mass of 30.7 KDa. It migrates as an approximately 37 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Leu 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for DNL1 recombinant protein

Background: DNase1, also known as deoxyribonuclease I and DNL1, is a member of the DNase family. DNaseI is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. DNaseI binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. Mutations in DNase1 gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. DNase1 is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity.

Description: A DNA sequence encoding the human DNASE1 (P24855) (Met1-Lys282) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 21361254
• NCBI GeneID: 1773
• NCBI Accession #: NP_005214.2
• NCBI GenBank Nucleotide #: NM_005223.3
• UniProt Accession #: P24855; Q14UU9; Q14UV0; B4DV35
• Molecular Weight: 19,771 Da
• NCBI Official Full Name: deoxyribonuclease-1
• NCBI Official Synonym Full Names: deoxyribonuclease 1
• NCBI Official Symbol: DNASE1
• NCBI Official Synonym Symbols: DNL1; DRNI
• NCBI Protein Information: deoxyribonuclease-1
• UniProt Protein Name: Deoxyribonuclease-1
• UniProt Gene Name: DNASE1
• UniProt Synonym Gene Names: DNL1; DRNI; DNase I

NCBI Description

This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family.

Protein type: Deoxyribonuclease; EC 3.1.21.1; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular region; nucleus

Molecular Function: protein binding

Disease: Systemic Lupus Erythematosus