Western Blot (WB)
(Western Blot analysis of extracts of various cells, using DNASE1 antibody.)
Rabbit anti-Human, Mouse DNASE1 Polyclonal Antibody | anti-DNASE1 antibody
DNASE1 Polyclonal Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
DNASE1; Polyclonal Antibody; DNASE1 Polyclonal Antibody; Deoxyribonuclease 1; Deoxyribonuclease I; Deoxyribonuclease-1; Deoxyribonuclease1; DeoxyribonucleaseI; DNAS1_HUMAN; DNASE 1; DNase I; DNase I lysosomal; DNaseI; DNL 1; DNL1; Dornase alfa; DRNI; FLJ38093; Human urine deoxyribonuclease I; anti-DNASE1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
DNASE1 polyclonal antibody detects endogenous levels of DNASE1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
282
Applicable Applications for anti-DNASE1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant full length Human DNASE1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using DNASE1 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using DNASE1 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cell using DNASE1 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cell using DNASE1 antibody.)
Related Product Information for anti-DNASE1 antibody
Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, and at least six alleles of DNase I are known. DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts. DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
~ 31kDa
NCBI Official Full Name
Deoxyribonuclease-1
UniProt Protein Name
Deoxyribonuclease-1
Protein Family
UniProt Gene Name
DNASE1
UniProt Synonym Gene Names
DNL1; DRNI; DNase I
UniProt Entry Name
DNAS1_HUMAN
Uniprot Description
DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family.
Protein type: Deoxyribonuclease; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; EC 3.1.21.1
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: extracellular region; nuclear envelope; nucleus
Molecular Function: protein binding; deoxyribonuclease I activity; actin binding; endodeoxyribonuclease activity
Biological Process: apoptosis; DNA catabolic process, endonucleolytic
Disease: Systemic Lupus Erythematosus
Similar Products
Product Notes
The DNASE1 dnase1 (Catalog #AAA3011262) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNASE1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DNASE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the DNASE1 dnase1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNASE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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