Western Blot (WB)
(Staining (2ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat KLF8/BKLF3 Polyclonal Antibody | anti-KLF8 antibody
Goat anti-KLF8/BKLF3 Antibody
Western Blot: Approx 50kDa band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2). In glioblastomas this protein was reported to run at appr. 60kDa (Ding et al. J Biol Chem.2005 Feb 25;280(8): 6802-15; PMID: 15557280). Recommended for use at 1-3ug/ml.
Western Blot (WB)
(Staining (2ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (2ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Uniprot Description
KLF8: Transcriptional repressor and activator. Binds to CACCC- boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK). Belongs to the Sp1 C2H2-type zinc-finger protein family.
Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: Xp11.21
Cellular Component: nucleus
Molecular Function: DNA binding; metal ion binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on KLF8
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Product Notes
The KLF8 klf8 (Catalog #AAA420636) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-KLF8/BKLF3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's KLF8/BKLF3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 50kDa band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2). In glioblastomas this protein was reported to run at appr. 60kDa (Ding et al. J Biol Chem.2005 Feb 25;280(8): 6802-15; PMID: 15557280). Recommended for use at 1-3ug/ml. Researchers should empirically determine the suitability of the KLF8 klf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: VDMDKLINNL EVQ. It is sometimes possible for the material contained within the vial of "KLF8/BKLF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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