Mouse Deiodinase, Iodothyronine, Type III (DIO3) ELISA Kit | DIO3 elisa kit

Mouse Deiodinase, Iodothyronine, Type III (DIO3) ELISA Kit

Cat. Num. AAA454151

• Reactivity: Mouse
• Synonyms: Deiodinase; Iodothyronine; Type III (DIO3); Mouse Deiodinase; Type III (DIO3) ELISA Kit; ITDI3; TXDI3; Type-III 5'-deiodinase; DIO3 elisa kit

• Reactivity: Mouse
• Sequence Length: 304

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.115ng/mL
• Intra Assay: CV<10%
• Inter Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 255958196
• NCBI GeneID: 107585
• NCBI Accession #: NP_742117.2
• NCBI GenBank Nucleotide #: NM_172119.2
• UniProt Accession #: Q91ZI8; Q3UKD2; G3UY28
• Molecular Weight: 34,111 Da
• NCBI Official Full Name: thyroxine 5-deiodinase
• NCBI Official Synonym Full Names: deiodinase, iodothyronine type III
• NCBI Official Symbol: Dio3
• NCBI Protein Information: thyroxine 5-deiodinase
• UniProt Protein Name: Thyroxine 5-deiodinase
• Protein Family: Thyroxine 5-deiodinase
• UniProt Gene Name: Dio3

NCBI Description

This is an intronless, imprinted gene that is preferentially expressed from the paternal allele in the mouse fetus. The encoded protein belongs to the iodothyronine deiodinase family, and catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5' triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. It is highly expressed in placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It thus plays a critical role in mammalian development by regulating circulating fetal thyroid hormone concentration. Knockout mice lacking this gene exhibit severe abnormalities related to development and reproduction. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2016]

Uniprot Description

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.