Mouse Deiodinase, Iodothyronine, Type III (DIO3) RTU ELISA Kit | DIO3 rtu elisa kit

Mouse Deiodinase, Iodothyronine, Type III (DIO3) ELISA Kit

Cat. Num. AAA458859

• Reactivity: Mouse
• Synonyms: Deiodinase; Iodothyronine; Type III (DIO3); Mouse Deiodinase; Type III (DIO3) ELISA Kit; ITDI3; TXDI3; Type-III 5'-deiodinase; DIO3 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 304

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.115ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DIO3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DIO3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DIO3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DIO3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DIO3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 255958196
• NCBI GeneID: 107585
• NCBI Accession #: NP_742117.2
• NCBI GenBank Nucleotide #: NM_172119.2
• UniProt Accession #: Q91ZI8; Q3UKD2; G3UY28
• Molecular Weight: 34,111 Da
• NCBI Official Full Name: thyroxine 5-deiodinase
• NCBI Official Synonym Full Names: deiodinase, iodothyronine type III
• NCBI Official Symbol: Dio3
• NCBI Protein Information: thyroxine 5-deiodinase
• UniProt Protein Name: Thyroxine 5-deiodinase
• Protein Family: Thyroxine 5-deiodinase
• UniProt Gene Name: Dio3

NCBI Description

This is an intronless, imprinted gene that is preferentially expressed from the paternal allele in the mouse fetus. The encoded protein belongs to the iodothyronine deiodinase family, and catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5' triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. It is highly expressed in placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It thus plays a critical role in mammalian development by regulating circulating fetal thyroid hormone concentration. Knockout mice lacking this gene exhibit severe abnormalities related to development and reproduction. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2016]

Uniprot Description

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.