Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cytochrome b5 (CYB5A) Standard Curve.)
Mouse Cytochrome B5 (CYB5A) ELISA Kit | CYB5A elisa kit
Mouse Cytochrome B5 (CYB5A) ELISA Kit
Gene Names
Cyb5a; Cyb5; 0610009N12Rik
Reactivity
Mouse
Synonyms
Cytochrome B5 (CYB5A); Mouse Cytochrome B5 (CYB5A) ELISA Kit; CYB5A; CYB5; MCB5; cytochrome b-5; cytochrome b5 (microsomal); microsomal cytochrome b5; type 1 cyt-b5; CYB5A elisa kit
Reactivity
Mouse
Specificity
Mouse Cytochrome b5 (CYB5A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CYB5A. No significant cross-reactivity or interference between Mouse CYB5A and analogues was observed.
Sequence Length
134
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cytochrome b5 (CYB5A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cytochrome b5 (CYB5A) Standard Curve.)
Related Product Information for CYB5A elisa kit
Description: This Mouse Cytochrome b5 (CYB5A) ELISA Kit employs a two-site sandwich ELISA to quantitate CYB5A in samples. An antibody specific for CYB5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYB5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYB5A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYB5A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: CYB5A (Cytochrome B5 Type A) is a Protein Coding gene. Diseases associated with CYB5A include Methemoglobinemia, Type Iv and 17-Alpha-Hydroxylase/17,20-Lyase Deficiency. Among its related pathways are Metabolism and Metabolism of water-soluble vitamins and cofactors. GO annotations related to this gene include enzyme binding and cytochrome-c oxidase activity. An important paralog of this gene is CYB5B. Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Background: CYB5A (Cytochrome B5 Type A) is a Protein Coding gene. Diseases associated with CYB5A include Methemoglobinemia, Type Iv and 17-Alpha-Hydroxylase/17,20-Lyase Deficiency. Among its related pathways are Metabolism and Metabolism of water-soluble vitamins and cofactors. GO annotations related to this gene include enzyme binding and cytochrome-c oxidase activity. An important paralog of this gene is CYB5B. Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cytochrome b5 isoform 1
NCBI Official Synonym Full Names
cytochrome b5 type A (microsomal)
NCBI Official Symbol
Cyb5a
NCBI Official Synonym Symbols
Cyb5; 0610009N12Rik
NCBI Protein Information
cytochrome b5
UniProt Protein Name
Cytochrome b5
Protein Family
UniProt Gene Name
Cyb5a
UniProt Synonym Gene Names
Cyb5
UniProt Entry Name
CYB5_MOUSE
Uniprot Description
CYB5A: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. Belongs to the cytochrome b5 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; intracellular membrane-bound organelle; membrane; mitochondrion
Molecular Function: enzyme binding; heme binding; metal ion binding; stearoyl-CoA 9-desaturase activity
Biological Process: fatty acid metabolic process; transport
Research Articles on CYB5A
Similar Products
Product Notes
The Mouse CYB5A cyb5a (Catalog #AAA9717975) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717975 ELISA Kit recognizes Mouse CYB5A. It is sometimes possible for the material contained within the vial of "Cytochrome B5 (CYB5A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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